PMID- 21784039
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110725
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 28
IP  - 3
DP  - 2009 Nov
TI  - Protective effect of Bojungbangdocktang on cisplatin-induced cytotoxicity and 
      apoptosis in MCF-10A breast endothelial cells.
PG  - 430-8
LID - 10.1016/j.etap.2009.07.007 [doi]
AB  - Although cisplatin has been extensively used as a cancer chemotherapeutic agent 
      for the treatment of various human cancers, it causes significant side effects 
      such as nephrotoxicity and hepatotoxicity due to lethal bystander damage to 
      normal cells. Thus, in the current study, we investigated the Oriental herbal 
      medicine Bojungbangdocktang (BJBDT), as we reported previously its 
      anti-angiogenic activity at nontoxic concentrations that could prevent 
      cisplatin-induced toxicity and apoptosis in human normal breast epithelial cell 
      MCF-10A, but not in MCF-7 and MDA MB-231 breast cancer cells. BJBDT protected 
      cisplatin-induced cytotoxicity in MCF-10A cells and potentiated cytotoxicity and 
      MMP loss in MCF-7 cells. Also, 4',6-diamidino-2-phenylindole (DAPI) staining and 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay revealed that BJBDT reduced cisplatin-induced apoptotic bodies in MCF-10A 
      cells compared with cisplatin-treated control. Consistently, BJBDT attenuated the 
      apoptotic portion sub-G1 DNA contents as well as blocked the activation of 
      caspase-3 and -9 and poly(ADP-ribose)polymerase (PARP) cleavage in 
      cisplatin-treated MCF-10A cells. Taken together, our findings suggest that BJBDT 
      can protect cisplatin-induced cytotoxicity and apoptosis in normal MCF-10A breast 
      cells as a cancer chemopreventive agent.
CI  - Copyright (c) 2009 Elsevier B.V. All rights reserved.
FAU - Kang, Sang-Hun
AU  - Kang SH
AD  - Cancer Preventive Material Development Research Center, Oriental Medical College, 
      Kyunghee University, 1 Hoegidong, Dongdaemungu, Seoul 130-701, South Korea.
FAU - Lee, Hyo-Jung
AU  - Lee HJ
FAU - Jeong, Soo-Jin
AU  - Jeong SJ
FAU - Kwon, Hee-Young
AU  - Kwon HY
FAU - Kim, Ji-Hyun
AU  - Kim JH
FAU - Yun, Sun-Mi
AU  - Yun SM
FAU - Kim, Jin-Hyung
AU  - Kim JH
FAU - Lee, Hyo-Jeong
AU  - Lee HJ
FAU - Lee, Eun-Ok
AU  - Lee EO
FAU - Ahn, Kwang Seok
AU  - Ahn KS
FAU - Ahn, Kyoo Seok
AU  - Ahn KS
FAU - Kim, Sung-Hoon
AU  - Kim SH
LA  - eng
PT  - Journal Article
DEP - 20090805
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
EDAT- 2009/11/01 00:00
MHDA- 2009/11/01 00:01
CRDT- 2011/07/26 06:00
PHST- 2009/04/23 00:00 [received]
PHST- 2009/07/08 00:00 [revised]
PHST- 2009/07/25 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2009/11/01 00:00 [pubmed]
PHST- 2009/11/01 00:01 [medline]
AID - S1382-6689(09)00137-9 [pii]
AID - 10.1016/j.etap.2009.07.007 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2009 Nov;28(3):430-8. doi: 10.1016/j.etap.2009.07.007. 
      Epub 2009 Aug 5.