PMID- 21784536
OWN - NLM
STAT- MEDLINE
DCOM- 20120217
LR  - 20111024
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 144
IP  - 1-2
DP  - 2011 Nov 15
TI  - Transplantation of CD6-depleted peripheral blood stem cells after 
      DLA-haploidentical bone marrow transplantation contributes to engraftment and 
      tolerance in a preclinical model of stem cell transplantation.
PG  - 27-35
LID - 10.1016/j.vetimm.2011.06.036 [doi]
AB  - Human leukocyte antigen (HLA)-haploidentical stem cell transplantation is an 
      opportunity for nearly all patients lacking an HLA matched stem cell donor. 
      However, graft rejection and graft-versus-host disease (GvHD) as well as 
      infectious complications still result in high treatment-related mortality. Here, 
      we used the dog as a preclinical model for the study of tolerance induction with 
      the aim to optimize and to improve a clinical protocol of haploidentical stem 
      cell transplantation. For this purpose CD6-depleted peripheral blood stem cells 
      (PBSCs) were transfused 6d after transplantation of unmodified bone marrow from 
      dog leukocyte antigen (DLA)-haploidentical littermate donors in order to induce 
      immune tolerance. Besides hematopoietic stem cells CD6-depleted PBSC contain, NK 
      cells and a minority of suppressive CD8-positive cells that may suppress 
      activated T lymphocytes. Recipients were conditioned with, cyclophosphamide and 
      antithymocyte globulin (ATG) preceded by a transfusion of donor buffy coat and 
      either 1, 2 or 3 x 3.3 Gy total body irradiation (TBI). Postgrafting 
      immunosuppression was limited to 30 d of cyclosporine and methotrexate. The 
      additional administration of CD6-depleted PBSCs after unmodified marrow could not 
      prevent GvHD, but it may improve engraftment and chimerism after conditioning 
      with 2 x 3.3 Gy TBI. Reasons for incomplete suppression and possible improvements 
      for clinical applications are discussed.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Zorn, Julia
AU  - Zorn J
AD  - Helmholtz Zentrum Munchen - National Research Center for Environmental Health, 
      Institute of Molecular Immunology, Clinical Cooperation Group Hematopoietic Cell 
      Transplantation, Marchioninistrasse 25, D-81377 Munich, Germany. 
      julia.zorn@helmholtz-muenchen.de
FAU - Schwamberger, Sabine
AU  - Schwamberger S
FAU - Panzer, Werner
AU  - Panzer W
FAU - Adler, Heiko
AU  - Adler H
FAU - Kolb, Hans-Jochem
AU  - Kolb HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110702
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD6 antigen)
SB  - IM
MH  - Animals
MH  - Antigens, CD/immunology
MH  - Antigens, Differentiation, T-Lymphocyte/immunology
MH  - Bone Marrow Transplantation/methods/*veterinary
MH  - Chimerism/veterinary
MH  - Colony-Forming Units Assay/veterinary
MH  - Disease Models, Animal
MH  - Dogs
MH  - Female
MH  - Graft vs Host Disease/immunology/prevention & control/veterinary
MH  - Hematopoietic Stem Cell Transplantation/methods/*veterinary
MH  - Male
EDAT- 2011/07/26 06:00
MHDA- 2012/02/18 06:00
CRDT- 2011/07/26 06:00
PHST- 2011/02/15 00:00 [received]
PHST- 2011/06/05 00:00 [revised]
PHST- 2011/06/27 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2011/07/26 06:00 [pubmed]
PHST- 2012/02/18 06:00 [medline]
AID - S0165-2427(11)00234-0 [pii]
AID - 10.1016/j.vetimm.2011.06.036 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2011 Nov 15;144(1-2):27-35. doi: 
      10.1016/j.vetimm.2011.06.036. Epub 2011 Jul 2.