PMID- 21785623
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Print)
IS  - 1741-427X (Linking)
VI  - 2011
DP  - 2011
TI  - In Vitro Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic 
      Acid-Rich Fraction from Typhonium flagelliforme Tuber.
PG  - 421894
LID - 10.1093/ecam/neq010 [doi]
LID - 421894
AB  - The plant Typhonium flagelliforme, commonly known as "rodent tuber" in Malaysia, 
      is often used as a health supplement and traditional remedy for alternative 
      cancer therapies, including leukemia. This study aimed to evaluate in vitro 
      anti-leukemic activity of dichloromethane extract/fraction number 7 (DCM/F7) from 
      T. flagelliforme tuber on human T4 lymphoblastoid (CEMss) cell line. The DCM 
      extract of tuber has been fractionated by column chromatography. The obtained 
      fractions were evaluated for its cytotoxicity toward CEMss cells as well as human 
      primary blood lymphocytes (PBLs). Assessment of apoptosis produced by the most 
      active fraction was evaluated by various microscopic techniques and further 
      confirmation of apoptosis was done by terminal deoxynucleotidyl transferase dUTP 
      nick end labeling (TUNEL) assay. Phytochemical screening was done by gas 
      chromatography-mass spectrometry (GC-MS). The results shows that 7 out of 12 
      fractions showed significant cytotoxicity against the selected cell line CEMss, 
      in which fractions DCM/F7, DCM/F11 and DCM/F12 showed exceptional activity with 
      3, 5 and 6.2 mug ml(-1), respectively. Further studies in the non-cancerous PBL 
      exhibited significant selectivity of DCM/F7 compared to other fractions. 
      Cytological observations showed chromatin condensation, cell shrinkage, 
      abnormalities of cristae, membrane blebbing, cytoplasmic extrusions and formation 
      of apoptotic bodies as confirmed collectively by double-staining of acridine 
      orange (AO)/propidium iodide (PI), SEM and TEM. In addition, DCM/F7 has increased 
      the cellular DNA breaks on treated cells. GC-MS revealed that DCM/F7 contains 
      linoleic acid, hexadecanoic acid and 9-hexadecanoic acid. The present results 
      indicate that T. flagelliforme possess a valuable anti-leukemic effect and was 
      able to produce distinctive morphological features of cell death that corresponds 
      to apoptosis.
FAU - Mohan, Syam
AU  - Mohan S
AD  - UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, 43400 UPM Serdang, 
      University Putra Malaysia, Malaysia.
FAU - Bustamam, Ahmad
AU  - Bustamam A
FAU - Ibrahim, Siddig
AU  - Ibrahim S
FAU - Al-Zubairi, Adel S
AU  - Al-Zubairi AS
FAU - Aspollah, Mohammed
AU  - Aspollah M
FAU - Abdullah, Rasedee
AU  - Abdullah R
FAU - Elhassan, Manal Mohamed
AU  - Elhassan MM
LA  - eng
PT  - Journal Article
DEP - 20110502
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC3135625
EDAT- 2011/07/26 06:00
MHDA- 2011/07/26 06:01
PMCR- 2011/05/02
CRDT- 2011/07/26 06:00
PHST- 2009/10/24 00:00 [received]
PHST- 2010/01/25 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2011/07/26 06:00 [pubmed]
PHST- 2011/07/26 06:01 [medline]
PHST- 2011/05/02 00:00 [pmc-release]
AID - 10.1093/ecam/neq010 [doi]
PST - ppublish
SO  - Evid Based Complement Alternat Med. 2011;2011:421894. doi: 10.1093/ecam/neq010. 
      Epub 2011 May 2.