PMID- 21789168
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 7
DP  - 2011
TI  - Spheres derived from lung adenocarcinoma pleural effusions: molecular 
      characterization and tumor engraftment.
PG  - e21320
LID - 10.1371/journal.pone.0021320 [doi]
LID - e21320
AB  - Malignant pleural effusions (MPEs) could represent an excellent source to culture 
      a wide variety of cancer cells from different donors. In this study, we set up 
      culture conditions for cancer cells deriving from MPEs of several patients 
      affected by the most frequent form of lung cancer, namely the subset of non small 
      cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which 
      account for approximately 40% of lung cancer cases. AdenoCa malignant pleural 
      effusions gave rise to in vitro cultures both in adherent and/or in spheroid 
      conditions in almost all cases analyzed. We characterized in greater detail two 
      samples which showed the most efficient propagation in vitro. In these samples we 
      also compared gene profiles of spheroid vs adherent cultures and identified a set 
      of differentially expressed genes. Finally we achieved efficient tumor 
      engraftment in recipient NOD/SCID mice, also upon inoculation of small number of 
      cells, thus suggesting indirectly the presence of tumor initiating cells.
FAU - Mancini, Rita
AU  - Mancini R
AD  - Department of Clinical and Molecular Medicine, University of Rome La Sapienza S 
      Andrea Hospital, Rome, Italy. rita.mancini@uniroma1.it
FAU - Giarnieri, Enrico
AU  - Giarnieri E
FAU - De Vitis, Claudia
AU  - De Vitis C
FAU - Malanga, Donatella
AU  - Malanga D
FAU - Roscilli, Giuseppe
AU  - Roscilli G
FAU - Noto, Alessia
AU  - Noto A
FAU - Marra, Emanuele
AU  - Marra E
FAU - Laudanna, Carmelo
AU  - Laudanna C
FAU - Zoppoli, Pietro
AU  - Zoppoli P
FAU - De Luca, Pasquale
AU  - De Luca P
FAU - Affuso, Andrea
AU  - Affuso A
FAU - Ruco, Luigi
AU  - Ruco L
FAU - Di Napoli, Arianna
AU  - Di Napoli A
FAU - Mesiti, Giuseppe
AU  - Mesiti G
FAU - Aurisicchio, Luigi
AU  - Aurisicchio L
FAU - Ricci, Alberto
AU  - Ricci A
FAU - Mariotta, Salvatore
AU  - Mariotta S
FAU - Pisani, Lara
AU  - Pisani L
FAU - Andreetti, Claudio
AU  - Andreetti C
FAU - Viglietto, Giuseppe
AU  - Viglietto G
FAU - Rendina, Erino A
AU  - Rendina EA
FAU - Giovagnoli, Maria Rosaria
AU  - Giovagnoli MR
FAU - Ciliberto, Gennaro
AU  - Ciliberto G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110718
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers, Tumor)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
SB  - IM
MH  - Adenocarcinoma/*genetics/*pathology
MH  - Adenocarcinoma of Lung
MH  - Aged
MH  - Aged, 80 and over
MH  - Aldehyde Dehydrogenase/metabolism
MH  - Animals
MH  - Biomarkers, Tumor/metabolism
MH  - Cell Adhesion
MH  - Cell Separation
MH  - Computational Biology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, Neoplasm/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Lung Neoplasms/*genetics/*pathology
MH  - Male
MH  - Mice
MH  - Mice, SCID
MH  - Middle Aged
MH  - Neoplastic Stem Cells/metabolism/pathology
MH  - Pleural Effusion, Malignant/*genetics/*pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spheroids, Cellular/*metabolism/*pathology
MH  - Tumor Cells, Cultured
MH  - *Xenograft Model Antitumor Assays
PMC - PMC3138755
COIS- Competing Interests: The co-author (Giuseppe Mesiti) is an employee of Charles 
      River. His role has been simply that of a scientific advisor for some of the 
      experiments conducted in vivo in mice at BIOGEM, a non-profit institution. 
      Authors CDV, GR, DM, EM, CL, PDL, AA, LA, and GV, are employees of Biogem 
      s.c.a.r.l. This does not alter the authors' adherence to all the PLoS ONE 
      policies on sharing data and materials.
EDAT- 2011/07/27 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/18
CRDT- 2011/07/27 06:00
PHST- 2010/12/28 00:00 [received]
PHST- 2011/05/30 00:00 [accepted]
PHST- 2011/07/27 06:00 [entrez]
PHST- 2011/07/27 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/18 00:00 [pmc-release]
AID - PONE-D-11-00572 [pii]
AID - 10.1371/journal.pone.0021320 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(7):e21320. doi: 10.1371/journal.pone.0021320. Epub 2011 Jul 18.