PMID- 21790012
OWN - NLM
STAT- MEDLINE
DCOM- 20110906
LR  - 20110727
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 45
IP  - 3
DP  - 2011 May-Jun
TI  - Expression patterns of two Arabidopsis endo-beta-1,4-glucanase genes (At3g43860, 
      At4g39000) in reproductive development.
PG  - 503-9
AB  - Endo-beta-1,4-D-glucanases (EGases) are a widespread and vital group of glycosyl 
      hydrolases that generally break the beta-1,4-glucosyl linkages. Studies of plant 
      EGases have mainly been concentrated on vegetative growth, while little is 
      currently known about their role in reproductive processes. Using the GUS 
      reporter aided analysis of promoter activities, we identified the expression 
      patterns of two putative Arabidopsis EGases genes (At3g43860 and At4g39000) whose 
      promoters conferred specific localization of the GUS activity in reproductive 
      organs. We found that At3g43860, which is similar to KOR in its protein 
      structural organization, is expressed in mature pollen and the pollen tube, 
      implying that it may have a role in pollen and pollen tube growth. At4g39000 was 
      found to be activated in the developing ovules and seeds, especially at the 
      micropylar end of the inner integuments and nucellus in a proximal-distal 
      pattern. Our results suggested that the two EGases play specific roles in 
      Arabidopsis sexual reproduction.
FAU - Xie, Xiao-Jun
AU  - Xie XJ
AD  - Institute of Cell Biology, Lanzhou University, Tanshui Road 222, Lanzhou 730000, 
      Gansu, PR China.
FAU - Huang, Jun-Jun
AU  - Huang JJ
FAU - Gao, Huan-Huan
AU  - Gao HH
FAU - Guo, Guang-Qin
AU  - Guo GQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Arabidopsis Proteins)
RN  - EC 3.2.1.4 (At3g43860 protein, Arabidopsis)
RN  - EC 3.2.1.4 (At4g39000 protein, Arabidopsis)
RN  - EC 3.2.1.4 (Cellulase)
SB  - IM
MH  - Arabidopsis/enzymology/*genetics/*growth & development
MH  - Arabidopsis Proteins/*genetics
MH  - Cellulase/*genetics
MH  - *Gene Expression Regulation, Developmental
MH  - *Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Plant
MH  - Oligonucleotide Array Sequence Analysis
MH  - Pollen/enzymology/genetics
MH  - Promoter Regions, Genetic
MH  - Reproduction/genetics
MH  - Seeds/enzymology/genetics
EDAT- 2011/07/28 06:00
MHDA- 2011/09/07 06:00
CRDT- 2011/07/28 06:00
PHST- 2011/07/28 06:00 [entrez]
PHST- 2011/07/28 06:00 [pubmed]
PHST- 2011/09/07 06:00 [medline]
PST - ppublish
SO  - Mol Biol (Mosk). 2011 May-Jun;45(3):503-9.