PMID- 21790325
OWN - NLM
STAT- MEDLINE
DCOM- 20120531
LR  - 20151119
IS  - 1557-7732 (Electronic)
IS  - 1080-7683 (Linking)
VI  - 28
IP  - 1
DP  - 2012 Feb
TI  - Protective effects of hydrogen saline on diabetic retinopathy in a 
      streptozotocin-induced diabetic rat model.
PG  - 76-82
LID - 10.1089/jop.2010.0129 [doi]
AB  - PURPOSE: Diabetic retinopathy is the leading cause of blindness in the working 
      population of the developed countries and also a significant cause of blindness 
      in the elderly. This study aimed at examining the protective effect of H(2) 
      saline on diabetic retinopathy in a streptozotocin-induced diabetic rat model. 
      METHODS: Sprague-Dawley male rats were divided into 3 groups as follows: (1) 
      nondiabetic control group (non-DM control); (2) diabetic control group (DM 
      control); and (3) diabetic rats receiving H(2) saline therapy (DM H(2) saline). 
      Rats in DM H(2) saline group were intraperitoneally injected with H(2) saturated 
      saline (5 mL/kg) every day for 4 weeks. Retinal vascular permeability was 
      assessed by measuring Evans blue leakage into the retina. Retinal apoptosis was 
      evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining and measuring caspase-3 activity. Retinal thickness was 
      observed by hematoxylin and eosin staining. RESULTS: Our results showed that H(2) 
      saline treatment could depress the caspase activity, reduce the retinal 
      apoptosis, and vascular permeability. The H(2) saline could also prominently 
      attenuate the retinal parenchyma thickening that resulted from diabetic 
      retinopathy. CONCLUSIONS: Our preliminary studies indicated that H(2) saline may 
      have potentials in the clinical treatment of diabetic retinopathy.
FAU - Xiao, Xiang
AU  - Xiao X
AD  - Department of Scientific Research, Changzheng Hospital, Second Military Medical 
      University , Shanghai, PR China.
FAU - Cai, Jiping
AU  - Cai J
FAU - Xu, Jiajun
AU  - Xu J
FAU - Wang, Ruobing
AU  - Wang R
FAU - Cai, Jianmei
AU  - Cai J
FAU - Liu, Yun
AU  - Liu Y
FAU - Xu, Weigang
AU  - Xu W
FAU - Sun, Xuejun
AU  - Sun X
FAU - Li, Runping
AU  - Li R
LA  - eng
PT  - Journal Article
DEP - 20110726
PL  - United States
TA  - J Ocul Pharmacol Ther
JT  - Journal of ocular pharmacology and therapeutics : the official journal of the 
      Association for Ocular Pharmacology and Therapeutics
JID - 9511091
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 5W494URQ81 (Streptozocin)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Capillary Permeability/drug effects
MH  - Caspase 3/metabolism
MH  - Diabetes Mellitus, Experimental/*complications
MH  - Diabetic Retinopathy/*prevention & control
MH  - Hydrogen/chemistry/*pharmacology
MH  - In Situ Nick-End Labeling
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/drug effects/pathology
MH  - Sodium Chloride/chemistry/*pharmacology
MH  - Streptozocin
EDAT- 2011/07/28 06:00
MHDA- 2012/06/01 06:00
CRDT- 2011/07/28 06:00
PHST- 2011/07/28 06:00 [entrez]
PHST- 2011/07/28 06:00 [pubmed]
PHST- 2012/06/01 06:00 [medline]
AID - 10.1089/jop.2010.0129 [doi]
PST - ppublish
SO  - J Ocul Pharmacol Ther. 2012 Feb;28(1):76-82. doi: 10.1089/jop.2010.0129. Epub 
      2011 Jul 26.