PMID- 21791037
OWN - NLM
STAT- MEDLINE
DCOM- 20111031
LR  - 20211020
IS  - 1475-2875 (Electronic)
IS  - 1475-2875 (Linking)
VI  - 10
DP  - 2011 Jul 26
TI  - Global proteomic analysis of plasma from mice infected with Plasmodium berghei 
      ANKA using two dimensional gel electrophoresis and matrix assisted laser 
      desorption ionization-time of flight mass spectrometry.
PG  - 205
LID - 10.1186/1475-2875-10-205 [doi]
AB  - BACKGROUND: A global proteomic strategy was used to identify proteins, which are 
      differentially expressed in the murine model of severe malaria in the hope of 
      facilitating future development of novel diagnostic, disease monitoring and 
      treatment strategies. METHODS: Mice (4-week-old CD1 male mice) were infected with 
      Plasmodium berghei ANKA strain, and infection allowed to establish until a 
      parasitaemia of 30% was attained. Total plasma and albumin depleted plasma 
      samples from infected and control (non-infected) mice were separated by 
      two-dimensional gel electrophoresis (2-DE). After staining, the gels were imaged 
      and differential protein expression patterns were interrogated using image 
      analysis software. Spots of interest were then digested using trypsin and the 
      proteins identified using matrix-assisted laser desorption and ionization-time of 
      flight (MALDI-TOF) mass spectrometry (MS) and peptide mass fingerprinting 
      software. RESULTS: Master gels of control and infected mice, and the 
      corresponding albumin depleted fractions exhibited distinctly different 2D 
      patterns comparing control and infected plasma, respectively. A wide range of 
      proteins demonstrated altered expression including; acute inflammatory proteins, 
      transporters, binding proteins, protease inhibitors, enzymes, cytokines, 
      hormones, and channel/receptor-derived proteins. CONCLUSIONS: Malaria-infection 
      in mice results in a wide perturbation of the host serum proteome involving a 
      range of proteins and functions. Of particular interest is the increased 
      secretion of anti-inflammatory and anti apoptotic proteins.
FAU - Gitau, Evelyn N
AU  - Gitau EN
AD  - KEMRI-Wellcome Trust Collaborative Programme, Centre for Geographic Medicine 
      Coast, Kilifi, Kenya. egitau@kilifi.kemri-wellcome.org
FAU - Kokwaro, Gilbert O
AU  - Kokwaro GO
FAU - Newton, Charles Rjc
AU  - Newton CR
FAU - Ward, Stephen A
AU  - Ward SA
LA  - eng
GR  - 050533/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110726
PL  - England
TA  - Malar J
JT  - Malaria journal
JID - 101139802
RN  - 0 (Blood Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Blood Proteins/*analysis
MH  - Disease Models, Animal
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Malaria/parasitology/*pathology
MH  - Male
MH  - Mice
MH  - Plasma/*chemistry
MH  - Plasmodium berghei/*pathogenicity
MH  - *Proteome
MH  - Rodent Diseases/parasitology/pathology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PMC - PMC3199904
EDAT- 2011/07/28 06:00
MHDA- 2011/11/01 06:00
PMCR- 2011/07/26
CRDT- 2011/07/28 06:00
PHST- 2011/01/25 00:00 [received]
PHST- 2011/07/26 00:00 [accepted]
PHST- 2011/07/28 06:00 [entrez]
PHST- 2011/07/28 06:00 [pubmed]
PHST- 2011/11/01 06:00 [medline]
PHST- 2011/07/26 00:00 [pmc-release]
AID - 1475-2875-10-205 [pii]
AID - 10.1186/1475-2875-10-205 [doi]
PST - epublish
SO  - Malar J. 2011 Jul 26;10:205. doi: 10.1186/1475-2875-10-205.