PMID- 21795064
OWN - NLM
STAT- MEDLINE
DCOM- 20120130
LR  - 20141120
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 56
IP  - 2
DP  - 2011 Nov
TI  - An in vitro evaluation of inflammation response of titanium functionalized with 
      heparin/fibronectin complex.
PG  - 208-17
LID - 10.1016/j.cyto.2011.06.020 [doi]
AB  - Immobilization of biomolecules with a variety of biological functions has been a 
      promising method to improve the biocompatibility of biomaterials. However, little 
      is known about their inflammatory property and cytotoxicity, which are both key 
      aspects to most biomaterials designed for tissue engineering applications and in 
      vivo implantation. In this in vitro study, heparin/fibronectin complex (Hep/Fn) 
      was coimmobilized onto titanium surface (HF-Ti), which had been proven to have 
      the properties of both anticoagulation and endothelialization in our previous 
      study. Fourier transform infrared (FTIR) spectroscopy and water contact angle 
      measurement were utilized to determine the surface chemical compositions and 
      physical properties. Toluidine Blue O (TBO) and immunochemistry methods were 
      performed to quantify the surface-immobilized heparin and fibronectin. The early 
      inflammatory responses elicited by pristine Ti and HF-Ti were investigated by 
      proinflammatory cytokine secretion of tumor necrosis factor-alpha (TNF-alpha) 
      released by attached peritoneal macrophages, monocyte chemoattractant protein-1 
      (MCP-1) and interleukin-1beta (IL-1beta) released by attached human umbilical vein 
      endothelial cells (ECs), respectively. Scanning electronic microscopy (SEM) and 
      immunofluorescence were employed to investigate the changes in macrophages and 
      ECs morphologies. The incubation period for both cells was 24h and the results 
      showed that HF-Ti revealed a weaker inflammatory response than pristine Ti, which 
      provoked a stronger inflammatory response and higher activation of macrophages. 
      Our data suggest that Hep/Fn coimmobilized biomaterials surface may develop to be 
      a new generation of biomaterials with both biocompatibility and anti-inflammatory 
      properties, especially for used as cardiovascular implants and in tissue 
      engineering applications.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Li, Guicai
AU  - Li G
AD  - Key Laboratory for Advanced Technologies of Materials, Ministry of Education, 
      Southwest Jiaotong University, 610031 Chengdu, PR China.
FAU - Yang, Ping
AU  - Yang P
FAU - Guo, Xiang
AU  - Guo X
FAU - Huang, Nan
AU  - Huang N
FAU - Shen, Ru
AU  - Shen R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110726
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Fibronectins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9005-49-6 (Heparin)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Fibronectins/*chemistry
MH  - Fluorescent Antibody Technique
MH  - Heparin/*chemistry
MH  - Humans
MH  - In Vitro Techniques
MH  - Inflammation/*chemically induced
MH  - Macrophages
MH  - Microscopy, Electron, Scanning
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Titanium/*adverse effects/chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2011/07/29 06:00
MHDA- 2012/01/31 06:00
CRDT- 2011/07/29 06:00
PHST- 2010/10/04 00:00 [received]
PHST- 2011/05/31 00:00 [revised]
PHST- 2011/06/27 00:00 [accepted]
PHST- 2011/07/29 06:00 [entrez]
PHST- 2011/07/29 06:00 [pubmed]
PHST- 2012/01/31 06:00 [medline]
AID - S1043-4666(11)00212-2 [pii]
AID - 10.1016/j.cyto.2011.06.020 [doi]
PST - ppublish
SO  - Cytokine. 2011 Nov;56(2):208-17. doi: 10.1016/j.cyto.2011.06.020. Epub 2011 Jul 
      26.