PMID- 21798203
OWN - NLM
STAT- MEDLINE
DCOM- 20111123
LR  - 20240109
IS  - 1876-7478 (Electronic)
IS  - 1874-7787 (Linking)
VI  - 3
IP  - 2
DP  - 2010 Jun
TI  - Heterogeneity of surface attached microbial communities from Sydney Harbour, 
      Australia.
PG  - 99-105
LID - 10.1016/j.margen.2010.08.001 [doi]
AB  - There is limited information on bacterial communities attached to marine 
      surfaces. These surface attached bacterial communities can vary at a micro scale 
      and these differences may be due to surface characteristics in marine 
      environments. The current study investigates the heterogeneity of bacterial 
      communities on five different marine invertebrates (Heliocidaris erythrogramma, 
      Austrocochlea concamerata, Crassostrea gigas, Dendrilla rosea, and Actinia 
      tenebrosa), the alga, Lobophora variegata and marine gravel from a 20 m x 20 m 
      quadrant in Camp Cove, Sydney Harbour, Australia. Terminal restriction fragment 
      length polymorphism (TRFLP) of 16S sequences showed that each surface contained 
      unique combinations of TRFLP fragment lengths. Phylogenetic analysis of random 
      clones picked from clone libraries constructed from the amplified 16S sequences 
      revealed that 16S sequences from the communities on different surfaces clustered 
      into distinct clades. None of the bacteria identified by 16S sequencing of the 
      whole (uncultured) microbial communities was detected after cultivation. Overall, 
      the study shows surface type plays a major role in shaping microbial communities 
      in marine environments.
CI  - Crown Copyright (c) 2010. Published by Elsevier B.V. All rights reserved.
FAU - Wilson, George S
AU  - Wilson GS
AD  - Department of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, 
      Australia.
FAU - Raftos, David A
AU  - Raftos DA
FAU - Gillings, Michael R
AU  - Gillings MR
FAU - Nair, Sham V
AU  - Nair SV
LA  - eng
SI  - GENBANK/FJ178003
SI  - GENBANK/FJ178004
SI  - GENBANK/FJ178005
SI  - GENBANK/FJ178006
SI  - GENBANK/FJ178007
SI  - GENBANK/FJ178008
SI  - GENBANK/FJ178009
SI  - GENBANK/FJ178010
SI  - GENBANK/FJ178011
SI  - GENBANK/FJ178012
SI  - GENBANK/FJ178013
SI  - GENBANK/FJ178014
SI  - GENBANK/FJ178015
SI  - GENBANK/FJ178016
SI  - GENBANK/FJ178017
SI  - GENBANK/FJ178018
SI  - GENBANK/FJ178019
SI  - GENBANK/FJ178020
SI  - GENBANK/FJ178021
SI  - GENBANK/FJ178022
SI  - GENBANK/FJ178023
SI  - GENBANK/FJ178024
SI  - GENBANK/FJ178025
SI  - GENBANK/FJ178026
SI  - GENBANK/FJ178027
SI  - GENBANK/FJ178028
SI  - GENBANK/FJ178029
SI  - GENBANK/FJ178030
SI  - GENBANK/FJ178031
SI  - GENBANK/FJ178032
SI  - GENBANK/FJ178033
SI  - GENBANK/FJ178034
SI  - GENBANK/FJ178035
SI  - GENBANK/FJ178036
SI  - GENBANK/FJ178037
SI  - GENBANK/FJ178038
SI  - GENBANK/FJ178039
SI  - GENBANK/FJ178040
SI  - GENBANK/FJ178041
SI  - GENBANK/FJ178042
SI  - GENBANK/FJ178043
SI  - GENBANK/FJ178044
SI  - GENBANK/FJ178045
SI  - GENBANK/FJ178046
SI  - GENBANK/FJ178047
SI  - GENBANK/FJ178048
SI  - GENBANK/FJ178049
SI  - GENBANK/FJ178050
SI  - GENBANK/FJ178051
SI  - GENBANK/FJ178052
SI  - GENBANK/FJ178053
SI  - GENBANK/FJ178054
SI  - GENBANK/FJ178055
SI  - GENBANK/FJ178056
SI  - GENBANK/FJ178057
SI  - GENBANK/FJ178058
SI  - GENBANK/FJ178059
SI  - GENBANK/FJ178060
SI  - GENBANK/FJ178061
SI  - GENBANK/FJ178062
SI  - GENBANK/FJ178063
SI  - GENBANK/FJ178064
SI  - GENBANK/FJ178065
SI  - GENBANK/FJ178066
SI  - GENBANK/FJ178067
SI  - GENBANK/FJ178068
SI  - GENBANK/FJ178069
SI  - GENBANK/FJ178070
SI  - GENBANK/FJ178071
SI  - GENBANK/FJ178072
SI  - GENBANK/FJ178073
SI  - GENBANK/FJ178074
SI  - GENBANK/FJ178075
SI  - GENBANK/FJ178076
SI  - GENBANK/FJ178077
SI  - GENBANK/FJ178078
SI  - GENBANK/FJ178079
SI  - GENBANK/FJ178080
SI  - GENBANK/FJ178081
SI  - GENBANK/FJ178082
SI  - GENBANK/FJ178083
SI  - GENBANK/FJ178084
SI  - GENBANK/FJ178085
SI  - GENBANK/FJ178086
SI  - GENBANK/FJ178087
SI  - GENBANK/FJ178088
SI  - GENBANK/FJ178089
SI  - GENBANK/FJ178090
SI  - GENBANK/FJ178091
SI  - GENBANK/FJ178092
SI  - GENBANK/FJ178093
SI  - GENBANK/FJ178094
SI  - GENBANK/FJ178095
SI  - GENBANK/FJ178096
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100906
PL  - Netherlands
TA  - Mar Genomics
JT  - Marine genomics
JID - 101475200
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*genetics
MH  - Base Sequence
MH  - Bayes Theorem
MH  - *Biota
MH  - Cluster Analysis
MH  - Geologic Sediments/*microbiology
MH  - Invertebrates/*microbiology
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - New South Wales
MH  - Oceans and Seas
MH  - Phaeophyceae/*microbiology
MH  - *Phylogeny
MH  - Polymorphism, Restriction Fragment Length/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Surface Properties
EDAT- 2011/07/30 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/07/30 06:00
PHST- 2010/03/02 00:00 [received]
PHST- 2010/08/03 00:00 [accepted]
PHST- 2011/07/30 06:00 [entrez]
PHST- 2011/07/30 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S1874-7787(10)00025-5 [pii]
AID - 10.1016/j.margen.2010.08.001 [doi]
PST - ppublish
SO  - Mar Genomics. 2010 Jun;3(2):99-105. doi: 10.1016/j.margen.2010.08.001. Epub 2010 
      Sep 6.