PMID- 21799800
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 7
DP  - 2011
TI  - Identification of the Arabidopsis REDUCED DORMANCY 2 gene uncovers a role for the 
      polymerase associated factor 1 complex in seed dormancy.
PG  - e22241
LID - 10.1371/journal.pone.0022241 [doi]
LID - e22241
AB  - The life of a plant is characterized by major phase transitions. This includes 
      the agriculturally important transitions from seed to seedling (germination) and 
      from vegetative to generative growth (flowering induction). In many plant 
      species, including Arabidopsis thaliana, freshly harvested seeds are dormant and 
      incapable of germinating. Germination can occur after the release of dormancy and 
      the occurrence of favourable environmental conditions. Although the hormonal 
      control of seed dormancy is well studied, the molecular mechanisms underlying the 
      induction and release of dormancy are not yet understood.In this study, we report 
      the cloning and characterization of the mutant reduced dormancy 2-1 (rdo2-1). We 
      found that RDO2 is allelic to the recently identified dormancy gene TFIIS, which 
      is a transcription elongation factor. HUB1, which was previously called RDO4, was 
      identified in the same mutagenesis screen for reduced dormancy as rdo2-1 and was 
      also shown to be involved in transcription elongation. The human homologues of 
      RDO2 and HUB1 interact with the RNA Polymerase II Associated Factor 1 Complex 
      (PAF1C). Therefore, we investigated the effect of other Arabidopsis PAF1C related 
      factors; VIP4, VIP5, ELF7, ELF8 and ATXR7 on seed dormancy. Mutations in these 
      genes resulted in reduced dormancy, similar to hub1-2 and rdo2-1. Consistent with 
      a role at the end of seed maturation, we found that HUB1, RDO2 and VIP5 are 
      upregulated during this developmental phase. Since mutants in PAF1C related 
      factors are also described to be early flowering, we conclude that these 
      components are involved in the regulation of both major developmental transitions 
      in the plant.
FAU - Liu, Yongxiu
AU  - Liu Y
AD  - Department of Plant Breeding and Genetics, Max Planck Institute for Plant 
      Breeding Research, Cologne, Germany. soppe@mpiz-koeln.mpg.de
FAU - Geyer, Regina
AU  - Geyer R
FAU - van Zanten, Martijn
AU  - van Zanten M
FAU - Carles, Annaick
AU  - Carles A
FAU - Li, Yong
AU  - Li Y
FAU - Horold, Anja
AU  - Horold A
FAU - van Nocker, Steven
AU  - van Nocker S
FAU - Soppe, Wim J J
AU  - Soppe WJ
LA  - eng
SI  - GEO/GSE28446
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110725
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Transcriptional Elongation Factors)
RN  - 0 (transcription factor S-II)
SB  - IM
MH  - Alleles
MH  - Arabidopsis/*genetics/metabolism/*physiology
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Plant/genetics
MH  - Humans
MH  - Mutation/genetics
MH  - Plant Dormancy/*genetics
MH  - Transcription, Genetic/genetics
MH  - Transcriptional Elongation Factors/*genetics/metabolism
MH  - Up-Regulation/genetics
PMC - PMC3143138
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/07/30 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/25
CRDT- 2011/07/30 06:00
PHST- 2011/04/06 00:00 [received]
PHST- 2011/06/17 00:00 [accepted]
PHST- 2011/07/30 06:00 [entrez]
PHST- 2011/07/30 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/25 00:00 [pmc-release]
AID - PONE-D-11-06107 [pii]
AID - 10.1371/journal.pone.0022241 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(7):e22241. doi: 10.1371/journal.pone.0022241. Epub 2011 Jul 25.