PMID- 21801373
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20211020
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 9
DP  - 2011 Jul 29
TI  - Effect of estradiol on proliferation and differentiation of side population 
      stem/progenitor cells from murine endometrium.
PG  - 103
LID - 10.1186/1477-7827-9-103 [doi]
AB  - BACKGROUND: In our previous study, endometrium side population cells (SP cells) 
      were isolated from postpartum murine uterus, and characterized by a heterogeneous 
      population of stem/progenitor cells. In this study, we investigated the effect of 
      estrogen on the proliferation and differentiation of SP cells. METHODS: SP and 
      non-SP cells of postpartum murine endometrium were isolated by DNA dye Hoechst 
      33342. The expression of estrogen receptor 1 (ESR1) was measured by reverse 
      transcription polymerase chain reaction (RT-PCR), Real-time PCR, Western blot, 
      immunofluorescence and immunohistochemistry. The proliferation and 
      differentiation of SP cells treated with different concentrations [10(-8) 
      M-10(-6) M] of estradiol (E2) and E2+ ICI182780 (Faslodex, inhibitor of ESR1) 
      were measured by 3-(4, 5-dimethylthiazoly1-2)-2,5-diphenyltetrazolium 
      bromide(MTT) and clonogenic assays. RESULTS: (1) SP cells expressed ESR1 at a 
      higher level than non-SP cells. (2) The level of E2 in the serum and the 
      expression of ESR1 in the uterus of postpartum murine changed in the same manner 
      with the ratio of SP cells to total uterus cells at a different postpartum time 
      point. ESR1, as ABCG2 is also predominantly located in the stroma and the 
      glandular epithelium of the uterus. (3) 10(-6) M E2 notably promoted the 
      proliferation of SP cells after treatment for 24 h. This effect could be 
      inhibited by ICI182780. E2 at the concentration of 10(-7) M or 10(-8) M was sent 
      to impair the large cloning efficiency (CE) of SP cells. CONCLUSIONS: The effect 
      of estrogen on the proliferation and differentiation of endometrium SP cells via 
      ESR1 was observed and it was in a concentration dependent fashion. Clearly, more 
      work is needed to understand the in vivo effect of E2 at the physiological 
      concentration on the differentiation of SP cells.
FAU - Xu, Jing
AU  - Xu J
AD  - State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive 
      Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, 
      China.
FAU - Hu, Fei-Fei
AU  - Hu FF
FAU - Cui, Yu-Gui
AU  - Cui YG
FAU - Luo, Jian
AU  - Luo J
FAU - Jiang, Chun-Yan
AU  - Jiang CY
FAU - Gao, Li
AU  - Gao L
FAU - Qian, Xiao-Qiao
AU  - Qian XQ
FAU - Mao, Yun-Dong
AU  - Mao YD
FAU - Liu, Jia-Yin
AU  - Liu JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110729
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Estrogen Receptor alpha)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Endometrium/cytology
MH  - Estradiol/blood/*pharmacology
MH  - Estrogen Receptor alpha/biosynthesis
MH  - Female
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Stem Cells/*drug effects
PMC - PMC3164598
EDAT- 2011/08/02 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/29
CRDT- 2011/08/02 06:00
PHST- 2011/01/20 00:00 [received]
PHST- 2011/07/29 00:00 [accepted]
PHST- 2011/08/02 06:00 [entrez]
PHST- 2011/08/02 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/29 00:00 [pmc-release]
AID - 1477-7827-9-103 [pii]
AID - 10.1186/1477-7827-9-103 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2011 Jul 29;9:103. doi: 10.1186/1477-7827-9-103.