PMID- 21802072
OWN - NLM
STAT- MEDLINE
DCOM- 20111209
LR  - 20191210
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 271
IP  - 1
DP  - 2011
TI  - The evaluation of survival and proliferation of lymphocytes in autologous mixed 
      leukocyte reaction with dendritic cells. The comparison of incorporation of 
      (3)H-thymidine and differential gating method.
PG  - 78-84
LID - 10.1016/j.cellimm.2011.06.006 [doi]
AB  - Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and 
      induction of antitumour response. The mixed leukocyte reaction (MLR) of 
      T-lymphocytes and DCs is essential instrument for immunological mechanisms 
      studies. Conventionally used method for determination of T-lymphocytes 
      proliferation, (3)H-thymidine incorporation, provides only general information. 
      The method of flow cytometry and differential gating seems to be more suitable 
      for quantitative and qualitative analysis of T-lymphocyte proliferation. It is 
      based on time limited acquisition of events and on its distribution according to 
      forward and side scatter values. We decided to compare these two methods and 
      determine mutual correlation and compatibility. Eleven patients were studied and 
      in all cases DCs promoted the survival and proliferation of both CD4 and CD8 
      lymphocytes. Both methods retained consistency with regard to survival and 
      proliferation of CD4/CD8 lymphocytes. However, the correlation of these methods 
      was not convincing. Therefore, both these methods might be used for evaluation of 
      MLR, but each of them gives specific and complementary information.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Vesela, Romana
AU  - Vesela R
AD  - 1st Department of Medicine, 1st Faculty of Medicine and General University 
      Hospital, Charles University, U Nemocnice 2, 128 08 Praha, Czech Republic. 
      romanavesela@seznam.cz
FAU - Dolezalova, Ludmila
AU  - Dolezalova L
FAU - Pytlik, Robert
AU  - Pytlik R
FAU - Rychtrmocova, Hana
AU  - Rychtrmocova H
FAU - Mareckova, Helena
AU  - Mareckova H
FAU - Trneny, Marek
AU  - Trneny M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110706
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, CD)
RN  - 0 (CD71 antigen)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, Transferrin)
RN  - 10028-17-8 (Tritium)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD/immunology/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism/pathology
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism/pathology
MH  - *Cell Proliferation
MH  - Cell Survival/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/pathology
MH  - Female
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/immunology/pathology
MH  - Lipopolysaccharide Receptors/immunology/metabolism
MH  - Lymphocyte Culture Test, Mixed/*methods
MH  - Lymphocytes/*immunology/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/immunology/metabolism/pathology
MH  - Receptors, Transferrin/immunology/metabolism
MH  - Reproducibility of Results
MH  - Thymidine/metabolism
MH  - Tritium
EDAT- 2011/08/02 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/08/02 06:00
PHST- 2010/11/10 00:00 [received]
PHST- 2011/05/24 00:00 [revised]
PHST- 2011/06/08 00:00 [accepted]
PHST- 2011/08/02 06:00 [entrez]
PHST- 2011/08/02 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - S0008-8749(11)00144-4 [pii]
AID - 10.1016/j.cellimm.2011.06.006 [doi]
PST - ppublish
SO  - Cell Immunol. 2011;271(1):78-84. doi: 10.1016/j.cellimm.2011.06.006. Epub 2011 
      Jul 6.