PMID- 21803942
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20200930
IS  - 1522-1539 (Electronic)
IS  - 0363-6135 (Linking)
VI  - 301
IP  - 4
DP  - 2011 Oct
TI  - Sex hormone control of left ventricular structure/function: mechanistic insights 
      using echocardiography, expression, and DNA methylation analyses in adult mice.
PG  - H1706-15
LID - 10.1152/ajpheart.00088.2011 [doi]
AB  - Calcium flux into and out of the sarco(endo)plasmic reticulum is vitally 
      important to cardiac function because the cycle of calcium entry and exit 
      controls contraction and relaxation. Putative estrogen and androgen consensus 
      binding sites near to a CpG island are present in the cardiac calsequestrin 2 
      (CSQ2) promoter. Cardiomyocytes express sex hormone receptors and respond to sex 
      hormones. We hypothesized that sex hormones control CSQ2 expression in 
      cardiomyocytes and so affect cardiac structure/function. Echocardiographic 
      analysis of male and female C57bl6n mice identified thinner walled and lighter 
      hearts in females and significant concentric remodeling after long-term 
      gonadectomy. CSQ2 and sodium-calcium exchanger-1 (NCX1) expression was 
      significantly increased in female compared with male hearts and decreased 
      postovariectomy. NCX1, but not CSQ2, expression was increased postcastration. 
      CSQ2 expression was reduced when H9c2 cells were cultured in hormone-deficient 
      media; increased when estrogen receptor-alpha (ERalpha), estrogen receptor-beta (ERbeta), or 
      androgen agonists were added; and increased in hearts from ERbeta-deficient mice. 
      CSQ2 expression was reduced in mice fed a diet low in the methyl donor folic acid 
      and in cells treated with 5-azadeoxycytidine suggesting an involvement of DNA 
      methylation. DNA methylation in CpG in the CSQ2 CpG island was significantly 
      different in males and females and was additionally changed postgonadectomy. 
      Expression of DNA methyltransferases 1, 3a, and 3b was unchanged. These studies 
      strongly link sex hormone-directed changes in CSQ2 expression to DNA methylation 
      with changed expression correlated with altered left ventricular structure and 
      function.
FAU - Sebag, Igal A
AU  - Sebag IA
AD  - Division of Cardiology, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, 
      Quebec, Canada.
FAU - Gillis, Marc-Antoine
AU  - Gillis MA
FAU - Calderone, Angelino
AU  - Calderone A
FAU - Kasneci, Amanda
AU  - Kasneci A
FAU - Meilleur, Melissa
AU  - Meilleur M
FAU - Haddad, Rami
AU  - Haddad R
FAU - Noiles, William
AU  - Noiles W
FAU - Patel, Bhavini
AU  - Patel B
FAU - Chalifour, Lorraine E
AU  - Chalifour LE
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110729
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 0 (Calsequestrin)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (NCX1 protein, mouse)
RN  - 0 (Sodium-Calcium Exchanger)
RN  - 0 (casq2 protein, mouse)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium/physiology
MH  - Calsequestrin/genetics/physiology
MH  - Cell Line
MH  - DNA Methylation
MH  - Echocardiography
MH  - Estrogen Receptor beta/genetics/physiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Gonadal Steroid Hormones/biosynthesis/genetics/*physiology
MH  - Homeostasis/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Molecular Sequence Data
MH  - Myocardium/cytology
MH  - Orchiectomy
MH  - Ovariectomy
MH  - Sodium-Calcium Exchanger/genetics
MH  - Ventricular Function, Left/*physiology
EDAT- 2011/08/02 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/08/02 06:00
PHST- 2011/08/02 06:00 [entrez]
PHST- 2011/08/02 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - ajpheart.00088.2011 [pii]
AID - 10.1152/ajpheart.00088.2011 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2011 Oct;301(4):H1706-15. doi: 
      10.1152/ajpheart.00088.2011. Epub 2011 Jul 29.