PMID- 2180653
OWN - NLM
STAT- MEDLINE
DCOM- 19900507
LR  - 20201226
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 11
IP  - 2
DP  - 1990
TI  - Flow cytometric analysis of recombinant murine GM-CSF (rmuGM-CSF) induced changes 
      in the distribution of specific cell populations in vivo.
PG  - 283-91
AB  - The changes in the distribution of granulocytes, monocytes, and lymphocytes in 
      various tissue compartments following subcutaneous (SC) administration of 
      recombinant murine GM-CSF (rmuGM-CSF) in vivo was determined by flow cytometry in 
      time course studies. Balb/c mice were given single, daily SC injections of 1 or 4 
      micrograms of rmuGM-CSF for 10 days. Flow cytometric analysis was performed on 
      bone marrow (BMC), peritoneal exudate (PEC), and peripheral blood (PBC) cell 
      preparations from mice treated for 1, 3, and 10 days. Dual fluorescence was 
      employed to gate on leukocytes (T200+) and analyze for Ig+, Thy 1.2+, MAC+, and 
      8C5+ (granulocytes) cells. The analyses indicated that SC-rmuGM-CSF increased the 
      percentage of 8C5+ cells in PBC after 1 day of treatment. However, significant 
      changes in the cell composition of PEC and BMC were not observed until day 10 of 
      treatment and included increases in 8C5+ cells and the myeloid cell population, 
      respectively. Side scatter analysis (cell density) of PBC and PEC indicated that 
      the percentage of the granulocytic cell population increased significantly in 
      rmuGM-CSF treated mice. The changes observed in PEC and BMC appeared to be 
      dose-related whereas those observed in PBC were not. These data clearly 
      demonstrate the utility of flow cytometric analyses for detecting selective 
      effects of cytokines on cell populations that are involved in host defense 
      mechanisms.
FAU - Pennline, K J
AU  - Pennline KJ
AD  - Department of Allergy and Inflammation, Schering-Plough Corporation, Bloomfield, 
      New Jersey 07003.
FAU - Pellerito, F
AU  - Pellerito F
FAU - DaFonseca, M
AU  - DaFonseca M
FAU - Monahan, P
AU  - Monahan P
FAU - Siegel, M I
AU  - Siegel MI
FAU - Smith, S R
AU  - Smith SR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Recombinant Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Blood Cells/*cytology/drug effects
MH  - Bone Marrow/drug effects
MH  - *Bone Marrow Cells
MH  - Colony-Stimulating Factors/*pharmacology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor
MH  - Growth Substances/*pharmacology
MH  - Light
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peritoneal Cavity/*cytology
MH  - Recombinant Proteins/pharmacology
MH  - Scattering, Radiation
MH  - Time Factors
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1002/cyto.990110209 [doi]
PST - ppublish
SO  - Cytometry. 1990;11(2):283-91. doi: 10.1002/cyto.990110209.