PMID- 2180683
OWN - NLM
STAT- MEDLINE
DCOM- 19900430
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 126
IP  - 4
DP  - 1990 Apr
TI  - Interleukin-1 inhibits the ovarian steroid-induced luteinizing hormone surge and 
      release of hypothalamic luteinizing hormone-releasing hormone in rats.
PG  - 2145-52
AB  - Interleukin-1 (IL-1), a polypeptide cytokine secreted by activated macrophages, 
      has been postulated as a chemical messenger between the immune and endocrine 
      systems. IL-1-immunopositive neurons and fibers have been visualized in the human 
      and rat hypothalamus, and IL-1 receptors are present in the rat brain. We have 
      examined the effects of human recombinant IL-1 (alpha- and beta-subtypes) on LH 
      release in vivo and hypothalamic LHRH release in vitro. Ovariectomized rats were 
      primed with estradiol benzoate, and progesterone was injected 48 h later to 
      elicit a LH surge in the afternoon. IL-1 alpha and IL-1 beta were injected either 
      intracerebroventricularly (icv) via a preimplanted cannula in the third ventricle 
      of the brain or iv. Systemic injection of IL-1 alpha or IL-1 beta (58.8 pmol at 
      1300 and 1500 h) failed to influence the afternoon LH surge seen in 
      saline-injected control rats. However, IL-1 beta (1.76 pmol) administered icv at 
      1300 and 1500 h or a single icv injection at 1300 h blocked the 
      progesterone-induced LH surge. Similar icv injections of IL-1 alpha also 
      significantly suppressed the afternoon LH surge compared to that in 
      saline-injected control rats. However, IL-1 alpha was relatively less effective 
      than the beta-subtype, since the LH surge was detected in some rats. To ascertain 
      whether suppression of the LH surge was due to inhibition of LHRH release, the 
      medial basal hypothalamus-preoptic area of estradiol 
      benzoate-progesterone-treated ovariectomized rats was incubated with and without 
      IL-1. Both IL-1 alpha and IL-1 beta, at concentrations of 0.1 nM and higher, 
      significantly suppressed LHRH release in vitro from the medial basal 
      hypothalamus-preoptic area. In contrast, IL-1 (10 nM) was completely ineffective 
      in suppressing LHRH release from the microdissected median eminence. These 
      results demonstrated an overall inhibitory effect of icv IL-1 on the LHRH-LH axis 
      and suggest that suppression of the steroid-induced LH surge by IL-1 may 
      primarily be due to inhibition of LHRH release at hypothalamic sites located 
      within the blood-brain barrier.
FAU - Kalra, P S
AU  - Kalra PS
AD  - Department of Obstetrics and Gynecology, University of Florida College of 
      Medicine, Gainesville 32610.
FAU - Sahu, A
AU  - Sahu A
FAU - Kalra, S P
AU  - Kalra SP
LA  - eng
GR  - HD-11362/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Interleukin-1)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Estradiol/pharmacology
MH  - Female
MH  - Gonadotropin-Releasing Hormone/*metabolism
MH  - Hypothalamus/*metabolism
MH  - Injections, Intraventricular
MH  - Interleukin-1/*pharmacology
MH  - Luteinizing Hormone/*metabolism
MH  - Progesterone/*physiology
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
AID - 10.1210/endo-126-4-2145 [doi]
PST - ppublish
SO  - Endocrinology. 1990 Apr;126(4):2145-52. doi: 10.1210/endo-126-4-2145.