PMID- 21809885
OWN - NLM
STAT- MEDLINE
DCOM- 20120125
LR  - 20240320
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 83
IP  - 18
DP  - 2011 Sep 15
TI  - Continuous signal enhancement for sensitive aptamer affinity probe 
      electrophoresis assay using electrokinetic concentration.
PG  - 7086-93
LID - 10.1021/ac201307d [doi]
AB  - We describe an electrokinetic concentration-enhanced aptamer affinity probe 
      electrophoresis assay to achieve highly sensitive and quantitative detection of 
      protein targets in a microfluidic device. The key weaknesses of aptamer as a 
      binding agent (weak binding strength/fast target dissociation) were counteracted 
      by continuous injection of fresh sample while band-broadening phenomena were 
      minimized due to self-focusing effects. With 30 min of continuous signal 
      enhancement, we can detect 4.4 pM human immunoglobulin E (IgE) and 9 pM human 
      immunodeficiency virus 1 reverse transcriptase (HIV-1 RT), which are among the 
      lowest limits of detection (LOD) reported. IgE was detected in serum sample with 
      a LOD of 39 pM due to nonspecific interactions between aptamers and serum 
      proteins. The method presented in this paper also has broad applicability to 
      improve sensitivities of various other mobility shift assays.
FAU - Cheow, Lih Feng
AU  - Cheow LF
AD  - Department of Electrical Engineering and Computer Science, Massachusetts 
      Institute of Technology, Cambridge, Massachusetts 02139, United States.
FAU - Han, Jongyoon
AU  - Han J
LA  - eng
GR  - R01 CA119402/CA/NCI NIH HHS/United States
GR  - R01 CA119402-05/CA/NCI NIH HHS/United States
GR  - CA 119402/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110817
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.7.- (reverse transcriptase, Human immunodeficiency virus 1)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Electrophoresis/*methods
MH  - HIV Reverse Transcriptase/analysis
MH  - HIV-1/enzymology
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Kinetics
MH  - Microfluidic Analytical Techniques/methods
PMC - PMC3179849
MID - NIHMS317358
EDAT- 2011/08/04 06:00
MHDA- 2012/01/26 06:00
PMCR- 2012/09/15
CRDT- 2011/08/04 06:00
PHST- 2011/08/04 06:00 [entrez]
PHST- 2011/08/04 06:00 [pubmed]
PHST- 2012/01/26 06:00 [medline]
PHST- 2012/09/15 00:00 [pmc-release]
AID - 10.1021/ac201307d [doi]
PST - ppublish
SO  - Anal Chem. 2011 Sep 15;83(18):7086-93. doi: 10.1021/ac201307d. Epub 2011 Aug 17.