PMID- 21809997
OWN - NLM
STAT- MEDLINE
DCOM- 20110927
LR  - 20220408
IS  - 1543-2165 (Electronic)
IS  - 0003-9985 (Linking)
VI  - 135
IP  - 8
DP  - 2011 Aug
TI  - Effect of heterogeneous distribution of monosomy 3 on prognosis in uveal 
      melanoma.
PG  - 1042-7
LID - 10.5858/2010-0477-OAR1 [doi]
AB  - CONTEXT: Fluorescence in situ hybridization (FISH) analyses on tumor sections and 
      on isolated nuclei showed that even low numbers of cells with monosomy of 
      chromosome 3 adversely affected survival. OBJECTIVE: To determine what percentage 
      of uveal melanoma cells with monosomy of chromosome 3 influences patient 
      mortality. DESIGN: To determine the presence of monosomy 3, karyotyping and FISH 
      on cultured cells and FISH on isolated nuclei were performed on 50 primary uveal 
      melanomas. Clinical and pathologic prognostic factors were assessed and compared 
      with 5-year survival data. Analyses were performed using Cox proportional hazards 
      test, log-rank analysis, sensitivity, specificity, and positive and negative 
      likelihood ratios. RESULTS: Combined karyotyping and FISH on cultured cells 
      showed monosomy 3 in 19 of 50 cases (38%), whereas determination of the monosomy 
      3 status by FISH on isolated nuclei with a threshold of 5% assigned 31 of 50 
      cases (62%) to the monosomy-3 category. When monosomy 3 on isolated nuclei with a 
      cutoff value of 5% was used, a significant difference in 5-year survival was 
      present (hazard ratio, 15.5; P = .007), indicating that monosomy 3 in greater 
      than 5% of tumor cells is related to death due to metastases. CONCLUSION: In 
      uveal melanoma, the presence of greater than 5% of cells with monosomy 3, as 
      determined by FISH on isolated nuclei, is associated with the development of 
      metastases within 5 years after enucleation.
FAU - Bronkhorst, Inge H G
AU  - Bronkhorst IH
AD  - Department of Ophthalmology, Leiden University Medical Center, the Netherlands.
FAU - Maat, Willem
AU  - Maat W
FAU - Jordanova, Ekaterina S
AU  - Jordanova ES
FAU - Kroes, Wilma G M
AU  - Kroes WG
FAU - Schalij-Delfos, Nicoline E
AU  - Schalij-Delfos NE
FAU - Luyten, Gregorius P M
AU  - Luyten GP
FAU - Jager, Martine J
AU  - Jager MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Pathol Lab Med
JT  - Archives of pathology & laboratory medicine
JID - 7607091
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Eye Enucleation
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Melanoma/genetics/mortality/*secondary/surgery
MH  - Middle Aged
MH  - *Monosomy
MH  - Neoplasm Staging
MH  - Netherlands/epidemiology
MH  - Prognosis
MH  - Survival Rate
MH  - Uveal Neoplasms/genetics/mortality/*pathology/surgery
EDAT- 2011/08/04 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/08/04 06:00
PHST- 2011/08/04 06:00 [entrez]
PHST- 2011/08/04 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - 10.1043/2010-0477-OAR1 [pii]
AID - 10.5858/2010-0477-OAR1 [doi]
PST - ppublish
SO  - Arch Pathol Lab Med. 2011 Aug;135(8):1042-7. doi: 10.5858/2010-0477-OAR1.