PMID- 21810083
OWN - NLM
STAT- MEDLINE
DCOM- 20120130
LR  - 20211020
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 78
IP  - 3
DP  - 2011 Sep
TI  - Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell 
      immunoglobulin-like receptor-ligand identification by real-time polymerase chain 
      reaction.
PG  - 185-94
LID - 10.1111/j.1399-0039.2011.01749.x [doi]
AB  - The effector function of natural killer (NK) cells is modulated by surface 
      expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that 
      interact with human leukocyte antigen (HLA) class I ligands. We describe the use 
      of real-time polymerase chain reaction (PCR) assays that allow easy and quick 
      detection of 16 KIR genes and the presence/absence of KIR-ligands based on 
      allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. 
      These methods overcome the tedious and expensive nature of conventional KIR 
      genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, 
      sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing 
      (SBT). Using these two cost-effective assays, we measured the frequencies of 
      KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited 
      in South Africa.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Hong, H A
AU  - Hong HA
AD  - AIDS Virus Research Unit, National Institute for Communicable Diseases, National 
      Health Laboratory Services, Johannesburg, South Africa.
FAU - Loubser, A S
AU  - Loubser AS
FAU - de Assis Rosa, D
AU  - de Assis Rosa D
FAU - Naranbhai, V
AU  - Naranbhai V
FAU - Carr, W
AU  - Carr W
FAU - Paximadis, M
AU  - Paximadis M
FAU - Lewis, D A
AU  - Lewis DA
FAU - Tiemessen, C T
AU  - Tiemessen CT
FAU - Gray, C M
AU  - Gray CM
LA  - eng
GR  - K01 TW007793/TW/FIC NIH HHS/United States
GR  - K01 TW007793-03/TW/FIC NIH HHS/United States
GR  - U19 AI051794/AI/NIAID NIH HHS/United States
GR  - K01-TW00703-03/TW/FIC NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA Antigens)
RN  - 0 (Receptors, KIR)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/genetics
MH  - DNA Primers
MH  - Genotype
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, KIR/*genetics
PMC - PMC3150492
MID - NIHMS311772
COIS- Author Statement We disclose that there are no conflicts of interest or any 
      commercial links pertinent to this article.
EDAT- 2011/08/04 06:00
MHDA- 2012/01/31 06:00
PMCR- 2012/09/01
CRDT- 2011/08/04 06:00
PHST- 2011/08/04 06:00 [entrez]
PHST- 2011/08/04 06:00 [pubmed]
PHST- 2012/01/31 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - 10.1111/j.1399-0039.2011.01749.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2011 Sep;78(3):185-94. doi: 10.1111/j.1399-0039.2011.01749.x.