PMID- 21810468
OWN - NLM
STAT- MEDLINE
DCOM- 20120402
LR  - 20111121
IS  - 1769-7123 (Electronic)
IS  - 0923-2508 (Linking)
VI  - 162
IP  - 9
DP  - 2011 Nov
TI  - Anaerobic oxidation of n-alkenes by sulphate-reducing bacteria from the genus 
      Desulfatiferula: n-ketones as potential metabolites.
PG  - 915-22
LID - 10.1016/j.resmic.2011.07.004 [doi]
AB  - Two alkene-degrading sulphate-reducing bacteria from the genus Desulfatiferula 
      (Desulfatiferula olefinivorans strain LM2801(T) and Desulfatiferula sp. strain 
      BE2801) were investigated for their 1-alkene metabolism. Their total cellular 
      fatty acids were predominantly C-even when they were grown on C-even 1-alkene 
      (1-hexadecene), whereas a mixture of fatty acids with C-odd or C-even carbon 
      chains predominated when cells were grown on C-odd 1-alkene (1-pentadecene). This 
      is consistent with the fatty acid composition of other sulphate-reducing strains 
      previously reported to grow on n-alkenes. Linear and 3-OH-fatty acids appear to 
      be the main fatty acids produced by the two Desulfatiferula strains. The analysis 
      of their neutral lipids led to identifying several n-alkanols and n-ketones with 
      the same number of carbon atoms as the alkene growth substrate and with 
      functionality located between C-1 and C-5. Growth of strains LM2801(T) and BE2801 
      on (per) deuterated 1-alkenes provided direct evidence of their anaerobic 
      transformation to corresponding 1-alkanols, n-ketones and linear (3-OH-) fatty 
      acids. These results demonstrate that Desulfatiferula strains oxidize a 1-alkene 
      by oxidation of the double bond at C-1, but also at C-2 to C-5 (after eventual 
      isomerization of the double bond) yielding the corresponding C-2 to C-5 n-ketones 
      (via the corresponding n-alkanols). The formation of specific 3-OH-fatty acids by 
      elongation of shorter chain fatty acids was also demonstrated. Based on our 
      observations, pathways for anaerobic 1-alkene metabolism in sulphate-reducing 
      bacteria from the genus Desulfatiferula are proposed. They indicate that 
      n-ketones can constitute new metabolites of the biodegradation of n-alkenes in 
      anaerobic environments.
CI  - Copyright (c) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Grossi, Vincent
AU  - Grossi V
AD  - Laboratoire de Geologie de Lyon (UMR 5276), CNRS, Universite Claude Bernard Lyon 
      1, Villeurbanne, France. vincent.grossi@univ-lyon1.fr
FAU - Cravo-Laureau, Cristiana
AU  - Cravo-Laureau C
FAU - Rontani, Jean-Francois
AU  - Rontani JF
FAU - Cros, Magali
AU  - Cros M
FAU - Hirschler-Rea, Agnes
AU  - Hirschler-Rea A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110720
PL  - France
TA  - Res Microbiol
JT  - Research in microbiology
JID - 8907468
RN  - 0 (Alkenes)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Ketones)
RN  - 0 (Sulfates)
SB  - IM
MH  - Alkenes/*metabolism
MH  - Anaerobiosis
MH  - Biodegradation, Environmental
MH  - Fatty Acids, Unsaturated/*metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Ketones/*metabolism
MH  - Lipid Metabolism
MH  - Oxidation-Reduction
MH  - Sulfates/metabolism
MH  - Sulfur-Reducing Bacteria/*metabolism
EDAT- 2011/08/04 06:00
MHDA- 2012/04/03 06:00
CRDT- 2011/08/04 06:00
PHST- 2010/12/15 00:00 [received]
PHST- 2011/06/22 00:00 [accepted]
PHST- 2011/08/04 06:00 [entrez]
PHST- 2011/08/04 06:00 [pubmed]
PHST- 2012/04/03 06:00 [medline]
AID - S0923-2508(11)00130-6 [pii]
AID - 10.1016/j.resmic.2011.07.004 [doi]
PST - ppublish
SO  - Res Microbiol. 2011 Nov;162(9):915-22. doi: 10.1016/j.resmic.2011.07.004. Epub 
      2011 Jul 20.