PMID- 21812961
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20211020
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 9
DP  - 2011 Aug 3
TI  - Prenatal testosterone-induced fetal growth restriction is associated with 
      down-regulation of rat placental amino acid transport.
PG  - 110
LID - 10.1186/1477-7827-9-110 [doi]
AB  - BACKGROUND: Exposure of pregnant mothers to elevated concentrations of 
      circulating testosterone levels is associated with fetal growth restriction and 
      delivery of small-for-gestational-age babies. We examined whether maternal 
      testosterone crosses the placenta to directly suppress fetal growth or if it 
      modifies placental function to reduce the capacity for transport of nutrients to 
      the fetus. METHODS: Pregnant rats were exposed to testosterone propionate (TP; 
      0.5 mg/kg) by daily subcutaneous injection from gestational days (GD) 15-19. 
      Maternal and fetal testosterone levels, placental nutrient transport activity and 
      expression of transporters and birth weight of pups and their anogenital 
      distances were determined. RESULTS: This dose of TP doubled maternal testosterone 
      levels but had no effect on fetal testosterone levels. Maternal daily weight gain 
      was significantly lower only on GD 19 in TP treated dams compared to controls. 
      Placental weight and birth weight of pups were significantly reduced, but the 
      anogenital distance of pups were unaffected by TP treatment. Maternal plasma 
      amino acids concentrations were altered following testosterone exposure, with 
      decreases in glutamine, glycine, tyrosine, serine, proline, and hydroxyproline 
      and increases in asparagine, isoleucine, leucine, lysine, histidine and arginine. 
      In the TP dams, placental system A amino acid transport activity was 
      significantly reduced while placental glucose transport capacity was unaffected. 
      Decreased expression of mRNA and protein levels of slc38a2/Snat2, an amino acid 
      transporter, suggests that reduced transporter proteins may be responsible for 
      the decrease in amino acid transport activity. CONCLUSIONS: Taken together, these 
      data suggest that increased maternal testosterone concentrations do not cross the 
      placenta to directly suppress fetal growth but affects amino acid nutrient 
      delivery to the fetus by downregulating specific amino acid transporter activity.
FAU - Sathishkumar, Kunju
AU  - Sathishkumar K
AD  - Department of Obstetrics and Gynecology, The University of Texas Medical Branch 
      Galveston, Texas, USA. kusathis@utmb.edu
FAU - Elkins, Rebekah
AU  - Elkins R
FAU - Chinnathambi, Vijayakumar
AU  - Chinnathambi V
FAU - Gao, Haijun
AU  - Gao H
FAU - Hankins, Gary D V
AU  - Hankins GD
FAU - Yallampalli, Chandra
AU  - Yallampalli C
LA  - eng
GR  - R03 HD069750/HD/NICHD NIH HHS/United States
GR  - HD069750/HD/NICHD NIH HHS/United States
GR  - HL72650/HL/NHLBI NIH HHS/United States
GR  - R01 HL119869/HL/NHLBI NIH HHS/United States
GR  - HL58144/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110803
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Amino Acid Transport System A)
RN  - 0 (Amino Acid Transport Systems)
RN  - 0 (Amino Acids)
RN  - 0 (Slc38a2 protein, rat)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Amino Acid Transport System A/biosynthesis
MH  - Amino Acid Transport Systems/*genetics
MH  - Amino Acids/blood/metabolism
MH  - Animals
MH  - Birth Weight/drug effects
MH  - Down-Regulation
MH  - Female
MH  - Fetal Growth Retardation/chemically induced/*physiopathology
MH  - Placenta/drug effects/physiopathology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testosterone/blood/*pharmacology
PMC - PMC3162507
EDAT- 2011/08/05 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/08/03
CRDT- 2011/08/05 06:00
PHST- 2011/04/13 00:00 [received]
PHST- 2011/08/03 00:00 [accepted]
PHST- 2011/08/05 06:00 [entrez]
PHST- 2011/08/05 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/08/03 00:00 [pmc-release]
AID - 1477-7827-9-110 [pii]
AID - 10.1186/1477-7827-9-110 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2011 Aug 3;9:110. doi: 10.1186/1477-7827-9-110.