PMID- 21813166
OWN - NLM
STAT- MEDLINE
DCOM- 20111215
LR  - 20111010
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 78
IP  - 4
DP  - 2011 Oct
TI  - Increased expression of hypoxia-inducible factor-1alpha and vascular endothelial 
      growth factor associated with glomerulation formation in patients with 
      interstitial cystitis.
PG  - 971.e11-5
LID - 10.1016/j.urology.2011.05.050 [doi]
AB  - OBJECTIVE: To examine whether hypoxia occurs in the bladders of patients with 
      interstitial cystitis (IC) by monitoring the expression of hypoxia-inducible 
      factor-1 (HIF-1) and VEGF. Previous studies have reported that bladder perfusion 
      is decreased in patients with IC. Hypoxia induces overexpression of vascular 
      endothelial growth factor (VEGF), which has been reported to be associated with 
      the formation of glomerulations in patients with IC. METHODS: The study group 
      consisted of 32 patients with IC, and the control group consisted of 8 
      volunteers. We obtained bladder biopsies from both groups and studied the 
      expression of HIF-1alpha and VEGF proteins by immunoblotting and immunohistochemical 
      and double immunofluorescent staining. Data were analyzed using the Mann-Whitney 
      U test. RESULTS: Immunoblotting and immunostaining revealed that the expression 
      of HIF-1alpha and VEGF proteins was increased in the study group compared with the 
      control group. The relative intensities of HIF-1alpha and VEGF proteins were 60.60 +/- 
      7.81 and 43.60 +/- 5.37 in the study group and 26.20 +/- 4.72 and 20.25 +/- 1.45 in the 
      control group, respectively. The overexpression of VEGF in study group biopsies 
      was particularly evident in umbrella cells examined by confocal microscopy. 
      CONCLUSION: Our findings identified increased expression of HIF-1alpha in bladder 
      tissue and overexpression of VEGF in umbrella cells from patients with IC. These 
      events may be associated with glomerulation formation during hydrodistention in 
      IC bladders. Thus, these molecular findings could offer the therapeutic mechanism 
      for hyperbaric oxygenation application to patients with IC.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Lee, Jane-Dar
AU  - Lee JD
AD  - Department of Surgery, Taichung Armed Forces General Hospital, Taiwan.
FAU - Lee, Ming-Huei
AU  - Lee MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110802
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Cystitis, Interstitial/*metabolism/pathology
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*biosynthesis
MH  - Kidney Glomerulus/*metabolism
MH  - Male
MH  - Microscopy, Confocal/methods
MH  - Microscopy, Fluorescence/methods
MH  - Middle Aged
MH  - Models, Biological
MH  - Urinary Bladder/pathology
MH  - Vascular Endothelial Growth Factor A/biosynthesis
EDAT- 2011/08/05 06:00
MHDA- 2011/12/16 06:00
CRDT- 2011/08/05 06:00
PHST- 2011/01/26 00:00 [received]
PHST- 2011/04/28 00:00 [revised]
PHST- 2011/05/26 00:00 [accepted]
PHST- 2011/08/05 06:00 [entrez]
PHST- 2011/08/05 06:00 [pubmed]
PHST- 2011/12/16 06:00 [medline]
AID - S0090-4295(11)00600-5 [pii]
AID - 10.1016/j.urology.2011.05.050 [doi]
PST - ppublish
SO  - Urology. 2011 Oct;78(4):971.e11-5. doi: 10.1016/j.urology.2011.05.050. Epub 2011 
      Aug 2.