PMID- 21813635
OWN - NLM
STAT- MEDLINE
DCOM- 20120402
LR  - 20111114
IS  - 1460-2407 (Electronic)
IS  - 1360-9947 (Linking)
VI  - 17
IP  - 12
DP  - 2011 Dec
TI  - Human leukocyte antigen-G in the male reproductive system and in seminal plasma.
PG  - 727-38
LID - 10.1093/molehr/gar052 [doi]
AB  - One of the non-classical human leukocyte antigen (HLA) class Ib proteins, HLA-G, 
      is believed to exert important immunoregulatory functions, especially during 
      pregnancy. The presence of HLA protein in paternal seminal fluid has been 
      suggested to have an influence on the risk of developing pre-eclampsia. We have 
      investigated whether HLA-G protein is present in human seminal plasma and in 
      different tissue samples of the male reproductive system. Western blot technique 
      and a soluble HLA-G (sHLA-G) assay were used to detect sHLA-G in human seminal 
      plasma samples. Immunohistochemical staining was performed on paraffin-embedded 
      tissue samples. We detected sHLA-G protein in seminal plasma, and HLA-G 
      expression in normal testis and in epididymal tissue of the male reproductive 
      system but not in the seminal vesicle. Furthermore, the results indicated a weak 
      expression of HLA-G in hyperplastic prostatic tissue. In summary, several of the 
      findings reported in this study suggest an immunoregulatory role of HLA-G in the 
      male reproductive system and in seminal plasma.
FAU - Larsen, Margit Horup
AU  - Larsen MH
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Copenhagen University Hospital (Roskilde) and Roskilde 
      Hospital, 7-13 Kogevej, DK-4000 Roskilde, Denmark.
FAU - Bzorek, Michael
AU  - Bzorek M
FAU - Pass, Malene B
AU  - Pass MB
FAU - Larsen, Lise Grupe
AU  - Larsen LG
FAU - Nielsen, Mette Weidinger
AU  - Nielsen MW
FAU - Svendsen, Signe Goul
AU  - Svendsen SG
FAU - Lindhard, Anette
AU  - Lindhard A
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110803
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (HLA-G Antigens)
SB  - IM
MH  - Blotting, Western
MH  - Endometrium/immunology/metabolism
MH  - Epididymis/immunology/metabolism
MH  - Female
MH  - HLA-G Antigens/analysis/*biosynthesis/immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Microtomy
MH  - Paraffin Embedding
MH  - Placenta/immunology/metabolism
MH  - Pre-Eclampsia/genetics/*immunology/metabolism
MH  - Pregnancy
MH  - Reproduction/genetics/*immunology
MH  - Risk Factors
MH  - Semen/immunology/*metabolism
MH  - Solubility
MH  - Testis/immunology/*metabolism
EDAT- 2011/08/05 06:00
MHDA- 2012/04/03 06:00
CRDT- 2011/08/05 06:00
PHST- 2011/08/05 06:00 [entrez]
PHST- 2011/08/05 06:00 [pubmed]
PHST- 2012/04/03 06:00 [medline]
AID - gar052 [pii]
AID - 10.1093/molehr/gar052 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2011 Dec;17(12):727-38. doi: 10.1093/molehr/gar052. Epub 2011 Aug 
      3.