PMID- 21814581
OWN - NLM
STAT- MEDLINE
DCOM- 20111208
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 7
DP  - 2011
TI  - Stage-specific histone modification profiles reveal global transitions in the 
      Xenopus embryonic epigenome.
PG  - e22548
LID - 10.1371/journal.pone.0022548 [doi]
LID - e22548
AB  - Vertebrate embryos are derived from a transitory pool of pluripotent cells. By 
      the process of embryonic induction, these precursor cells are assigned to 
      specific fates and differentiation programs. Histone post-translational 
      modifications are thought to play a key role in the establishment and maintenance 
      of stable gene expression patterns underlying these processes. While on gene 
      level histone modifications are known to change during differentiation, very 
      little is known about the quantitative fluctuations in bulk histone modifications 
      during development. To investigate this issue we analysed histones isolated from 
      four different developmental stages of Xenopus laevis by mass spectrometry. In 
      toto, we quantified 59 modification states on core histones H3 and H4 from 
      blastula to tadpole stages. During this developmental period, we observed in 
      general an increase in the unmodified states, and a shift from histone 
      modifications associated with transcriptional activity to transcriptionally 
      repressive histone marks. We also compared these naturally occurring patterns 
      with the histone modifications of murine ES cells, detecting large differences in 
      the methylation patterns of histone H3 lysines 27 and 36 between pluripotent ES 
      cells and pluripotent cells from Xenopus blastulae. By combining all detected 
      modification transitions we could cluster their patterns according to their 
      embryonic origin, defining specific histone modification profiles (HMPs) for each 
      developmental stage. To our knowledge, this data set represents the first 
      compendium of covalent histone modifications and their quantitative flux during 
      normogenesis in a vertebrate model organism. The HMPs indicate a stepwise 
      maturation of the embryonic epigenome, which may be causal to the progressing 
      restriction of cellular potency during development.
FAU - Schneider, Tobias D
AU  - Schneider TD
AD  - Department of Molecular Biology, Adolf-Butenandt Institut, 
      Ludwig-Maximilians-Universitat Munchen, Munich, Germany.
FAU - Arteaga-Salas, Jose M
AU  - Arteaga-Salas JM
FAU - Mentele, Edith
AU  - Mentele E
FAU - David, Robert
AU  - David R
FAU - Nicetto, Dario
AU  - Nicetto D
FAU - Imhof, Axel
AU  - Imhof A
FAU - Rupp, Ralph A W
AU  - Rupp RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110722
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Histones)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animals
MH  - Blastula/cytology/metabolism
MH  - Blotting, Western
MH  - Cell Differentiation
MH  - Chromatography, Liquid
MH  - Embryo, Nonmammalian/cytology/*metabolism
MH  - Embryonic Stem Cells/*metabolism
MH  - *Epigenomics
MH  - Gene Expression Profiling
MH  - Histones/*chemistry/metabolism
MH  - Lysine/chemistry/genetics
MH  - Methylation
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Protein Processing, Post-Translational
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Xenopus laevis/*embryology/*genetics
PMC - PMC3142184
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/08/05 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/22
CRDT- 2011/08/05 06:00
PHST- 2011/01/26 00:00 [received]
PHST- 2011/06/29 00:00 [accepted]
PHST- 2011/08/05 06:00 [entrez]
PHST- 2011/08/05 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/22 00:00 [pmc-release]
AID - PONE-D-11-01992 [pii]
AID - 10.1371/journal.pone.0022548 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(7):e22548. doi: 10.1371/journal.pone.0022548. Epub 2011 Jul 22.