PMID- 21814704
OWN - NLM
STAT- MEDLINE
DCOM- 20111220
LR  - 20151119
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 11
IP  - 18
DP  - 2011 Sep 21
TI  - A PDMS-based biochip with integrated sub-micrometre position control for TIRF 
      microscopy of the apical cell membrane.
PG  - 3064-71
LID - 10.1039/c1lc20458k [doi]
AB  - A poly(dimethylsiloxane) (PDMS)-based biochip with an integrated pressure 
      controlled positioning system with sub-micrometre precision was realized. The 
      biochip was easy and cheap to manufacture and enabled positioning in a wet 
      environment. It allowed the application of total internal reflection fluorescence 
      (TIRF) microscopy at the dorsal cell membrane, which is not adhering to a 
      support. Specifically, the chip enabled TIRF microscopy at the apical membrane of 
      polarized epithelial cells. Thereby, the device allowed us for the first time to 
      monitor individual fusion events of GPI-GFP bearing vesicles at the apical 
      membrane in live Madin-Darby canine kidney II (MDCK II) cells. Moreover, a 
      mapping of fusion sites became feasible and revealed that the whole apical 
      membrane is fusion competent. In total, the biochip offers an all-in-one solution 
      for apical TIRF microscopy and contributes a novel tool to study trafficking 
      processes close to the apical plasma membrane in polarized epithelial cells.
CI  - This journal is (c) The Royal Society of Chemistry 2011
FAU - Thuenauer, Roland
AU  - Thuenauer R
AD  - Center for Advanced Bioanalysis GmbH, Scharitzerstrasse 6-8, 4020, Linz, Austria. 
      roland.thuenauer@cbl.at
FAU - Juhasz, Kata
AU  - Juhasz K
FAU - Mayr, Reinhard
AU  - Mayr R
FAU - Fruhwirth, Thomas
AU  - Fruhwirth T
FAU - Lipp, Anna-Maria
AU  - Lipp AM
FAU - Balogi, Zsolt
AU  - Balogi Z
FAU - Sonnleitner, Alois
AU  - Sonnleitner A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110804
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Membrane Proteins)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/metabolism/*ultrastructure
MH  - Cell Polarity
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Dogs
MH  - Epithelial Cells/metabolism/ultrastructure
MH  - Equipment Design
MH  - Image Processing, Computer-Assisted
MH  - *Lab-On-A-Chip Devices
MH  - Membrane Fusion
MH  - Membrane Proteins/metabolism
MH  - Microchip Analytical Procedures/methods
MH  - Microscopy, Fluorescence/*instrumentation
MH  - Pressure
MH  - Single-Cell Analysis/*instrumentation/methods
MH  - Transport Vesicles
EDAT- 2011/08/05 06:00
MHDA- 2011/12/21 06:00
CRDT- 2011/08/05 06:00
PHST- 2011/08/05 06:00 [entrez]
PHST- 2011/08/05 06:00 [pubmed]
PHST- 2011/12/21 06:00 [medline]
AID - 10.1039/c1lc20458k [doi]
PST - ppublish
SO  - Lab Chip. 2011 Sep 21;11(18):3064-71. doi: 10.1039/c1lc20458k. Epub 2011 Aug 4.