PMID- 21815187
OWN - NLM
STAT- MEDLINE
DCOM- 20120406
LR  - 20111025
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 112
IP  - 12
DP  - 2011 Dec
TI  - Reciprocal osteoblastic and osteoclastic modulation in co-cultured MG63 
      osteosarcoma cells and human osteoclast precursors.
PG  - 3704-13
LID - 10.1002/jcb.23295 [doi]
AB  - Osteosarcoma is usually associated with a disturbed bone metabolism. The aim of 
      this work was to characterize the reciprocal interactions between MG63 
      osteosarcoma cells and osteoclasts, in a co-culture system. Co-cultures were 
      characterized throughout 21 days for the osteoclastogenic response and the 
      expression of osteoblastic markers. Monocultures of MG63 cells and peripheral 
      blood mononuclear cell (PBMC) and co-cultures of PBMC + human bone marrow cells 
      (hBMC) were also performed. Compared to PBMC cultures, co-cultures yielded 
      significantly increased gene expression of osteoclast-related markers, 
      tartarate-acid resistant phosphatase (TRAP) activity, TRAP-positive 
      multinucleated cells, cells with actin rings and vitronectin receptors (VNR) and 
      calcitonin receptors (CTR) and calcium phosphate resorbing ability. Results 
      showed that the development of functional osteoclasts required a very low number 
      of MG63 cells, suggesting a high osteoclastogenic-triggering capacity of this 
      cell line. Subjacent mechanisms involved the pathways MEK and NF-kB, although 
      with a lower relevance than that observed on PBMC monocultures or co-cultures of 
      hBMC + PBMC; PGE2 production also had a contribution. Compared to MG63 cell 
      monocultures, the co-culture expressed lower levels of COL1 and ALP, and higher 
      levels of BMP-2, suggesting that PBMC also modulated the osteoblastic behavior. 
      While M-CSF appeared to be involved in the osteoclastogenic response on the MG63 
      + PBMC co-cultures, RANKL does not seem to be a key player in the process. On the 
      other hand, sphingosine-1-phosphate production might contribute to the modulation 
      of the osteoblastic behavior. Results suggest that the reciprocal modulation 
      between osteosarcoma and osteoclastic cells might contribute to the disturbed 
      bone metabolism associated with bone tumors.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Costa-Rodrigues, Joao
AU  - Costa-Rodrigues J
AD  - Laboratorio de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina 
      Dentaria, Universidade do Porto, Porto, Portugal.
FAU - Fernandes, Anabela
AU  - Fernandes A
FAU - Fernandes, Maria H
AU  - Fernandes MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - Bone Neoplasms/*pathology
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - DNA Primers
MH  - Humans
MH  - Osteoblasts/*cytology
MH  - Osteoclasts/*cytology
MH  - Osteosarcoma/*pathology
MH  - Polymerase Chain Reaction
EDAT- 2011/08/05 06:00
MHDA- 2012/04/07 06:00
CRDT- 2011/08/05 06:00
PHST- 2011/08/05 06:00 [entrez]
PHST- 2011/08/05 06:00 [pubmed]
PHST- 2012/04/07 06:00 [medline]
AID - 10.1002/jcb.23295 [doi]
PST - ppublish
SO  - J Cell Biochem. 2011 Dec;112(12):3704-13. doi: 10.1002/jcb.23295.