PMID- 21816405
OWN - NLM
STAT- MEDLINE
DCOM- 20120423
LR  - 20181201
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1228
DP  - 2012 Mar 9
TI  - Preparation of methacrylate-based monolith for capillary hydrophilic interaction 
      chromatography and its application in determination of nucleosides in urine.
PG  - 183-92
LID - 10.1016/j.chroma.2011.07.061 [doi]
AB  - A novel poly(N-acryloyltris(hydroxymethyl)aminomethane-co-pentaerythritol 
      triacrylate) (NAHAM-co-PETA) monolith was prepared in the 100 mum i.d. capillary 
      and investigated for capillary liquid chromatography (cLC). The polymer monolith 
      was synthesized by in situ polymerization of NAHAM and PETA in the presence of 
      polyethylene glycol (PEG) in dimethyl sulfoxide (DMSO) as the porogen. The porous 
      structure of monolith was optimized by changing the ratio of NAHAM to PETA, the 
      molecular weight and amount of PEG. To evaluate the separation performance of the 
      resultant polymer monolith, several groups of model compounds (including 
      nucleosides, benzoic acids and anilines) were selected to perform cLC separation. 
      Our results showed that these model compounds can be baseline separated on the 
      resultant poly(NAHAM-co-PETA) monolithic column with the optimized mobile phases. 
      The column efficiency was estimated to be 87,000 plates/m for acrylamide. In 
      addition, this monolithic column was coupled with on-line solid-phase 
      microextraction (SPME) for the analysis of four nucleosides (uridine, adenosine, 
      cytidine, guanosine) in urine. The limit of detection of the proposed method was 
      in the range from 40 to 52 ng/mL. The method reproducibility was obtained by 
      evaluating the intra- and inter-day precisions with relative standard deviations 
      (RSDs) less than 8.3% and 10.2%, respectively. Recoveries of the target analytes 
      from spiked urine samples were ranged from 86.5% to 106.8%.
CI  - Copyright A(c) 2011 Elsevier B.V. All rights reserved.
FAU - Chen, Ming-Luan
AU  - Chen ML
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Wei, Shan-Shan
AU  - Wei SS
FAU - Yuan, Bi-Feng
AU  - Yuan BF
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110725
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acrylates)
RN  - 0 (Aniline Compounds)
RN  - 0 (Benzoates)
RN  - 0 (Nucleosides)
RN  - 0 (Propylene Glycols)
RN  - 0 (poly(N-acryloyltris(hydroxymethyl)aminomethane))
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - PJJ1161ULF (pentaerythrityl triacrylate)
SB  - IM
MH  - Acrylates/*chemistry
MH  - Aniline Compounds/chemistry/isolation & purification
MH  - Benzoates/chemistry/isolation & purification
MH  - Chromatography, Liquid/*instrumentation/methods
MH  - Equipment Reuse
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Linear Models
MH  - Models, Chemical
MH  - Neoplasms
MH  - Nucleosides/chemistry/isolation & purification/*urine
MH  - Polyethylene Glycols
MH  - Porosity
MH  - Propylene Glycols/*chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Microextraction
EDAT- 2011/08/06 06:00
MHDA- 2012/04/24 06:00
CRDT- 2011/08/06 06:00
PHST- 2011/03/19 00:00 [received]
PHST- 2011/06/16 00:00 [revised]
PHST- 2011/07/17 00:00 [accepted]
PHST- 2011/08/06 06:00 [entrez]
PHST- 2011/08/06 06:00 [pubmed]
PHST- 2012/04/24 06:00 [medline]
AID - S0021-9673(11)01071-5 [pii]
AID - 10.1016/j.chroma.2011.07.061 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Mar 9;1228:183-92. doi: 10.1016/j.chroma.2011.07.061. Epub 
      2011 Jul 25.