PMID- 21819344
OWN - NLM
STAT- MEDLINE
DCOM- 20111212
LR  - 20171116
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 89
IP  - 4
DP  - 2011 Aug
TI  - OxLDL-mediated survival of macrophages does not require LDL internalization or 
      signalling by major pattern recognition receptors.
PG  - 387-95
AB  - Macrophages play a key role in the pathogenesis of atherosclerosis, in part by 
      destabilizing plaques. We and others have shown that low concentrations of 
      oxidized LDL (oxLDL) inhibit macrophage apoptosis. As oxLDL is present in 
      lesions, this may be a mechanism by which macrophage populations in the intima 
      are expanded. We have previously shown that oxLDL activates prosurvival 
      signalling pathways such as the phosphoinositide 3-kinase (PI3K) pathway in bone 
      marrow derived macrophages (BMDMs). However, little is known about more upstream 
      signalling events especially at the receptor level. The endocytic pattern 
      recognition receptors (PRRs), scavenger receptor A (SR-A) and CD36, are the main 
      receptors on macrophages for uptake of oxLDL and are therefore important in foam 
      cell formation. The signalling PRRs such as toll-like receptor (TLR) 2 and 4 also 
      bind some types of oxLDL. This study was done to determine if any of the known 
      PRRs are required for the anti-apoptotic effects of oxLDL in BMDMs. To do this, 
      we tested the effect of oxLDL on viability of BMDMs lacking both SR-A and CD36 or 
      lacking TLR2, TLR4, CD14, FcgammaRIIb, or RAGE. Our results indicate that none of 
      these receptors are essential for activating the oxLDL prosurvival pathway. 
      Furthermore, we show that the anti-apoptotic effect is not dependent on the 
      uptake of oxLDL.
FAU - Riazy, Maziar
AU  - Riazy M
AD  - Department of Medicine, University of British Columbia, Vancouver, Canada. 
      mriazy@interchange.ubc.ca
FAU - Chen, Johnny H
AU  - Chen JH
FAU - Yamamato, Yasuhiko
AU  - Yamamato Y
FAU - Yamamato, Hiroshi
AU  - Yamamato H
FAU - Duronio, Vincent
AU  - Duronio V
FAU - Steinbrecher, Urs P
AU  - Steinbrecher UP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (CD36 Antigens)
RN  - 0 (Fcgr2b protein, mouse)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Scavenger Receptors, Class A)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - CD36 Antigens/genetics
MH  - *Cell Survival
MH  - Cells, Cultured
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Lipoproteins, LDL/metabolism/*pharmacology/physiology
MH  - Macrophages/drug effects/metabolism/*physiology
MH  - Mice
MH  - Mice, Knockout
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, IgG/metabolism
MH  - Receptors, Immunologic/metabolism
MH  - Scavenger Receptors, Class A/genetics
MH  - *Signal Transduction
MH  - Toll-Like Receptors/*metabolism
EDAT- 2011/08/09 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/08/09 06:00
PHST- 2011/08/09 06:00 [entrez]
PHST- 2011/08/09 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - 10.1139/o11-035 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2011 Aug;89(4):387-95. doi: 10.1139/o11-035.