PMID- 21819759
OWN - NLM
STAT- MEDLINE
DCOM- 20111202
LR  - 20110808
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 25
IP  - 4
DP  - 2011 Jul-Aug
TI  - Transforming growth factor beta: a role in the upper airway and 
      rhinosinusitis-Dermatophagoides pteronyssinus-induced apoptosis with pulmonary 
      alveolar cells.
PG  - 231-5
LID - 10.2500/ajra.2011.25.3629 [doi]
AB  - BACKGROUND: There is a link with the upper and lower airway and disruption of 
      alveolar epithelial cells, which is a potential trigger for the reactivation of 
      the epithelial-mesenchymal trophic unit (EMTU) and induced characteristic airway 
      changes associated with allergic asthma. Dermatophagoides pteronyssinus is a 
      common inhalant indoor allergen and is known for causing allergic rhinitis and 
      airway inflammation. Transforming growth factor beta 1 (TGF-beta1) is a major 
      participant in the airway remodeling of asthma, a component of cellular stress 
      response pathways, and enhanced epithelial immunoreactivity is known to occur in 
      allergic rhinitis. METHODS: In this study, we show the ability of D. 
      pteronyssinus allergens from dialyzed standardized immunotherapy extract to 
      induce apoptosis and increase TGF-beta1 secretion in a confluent A549 cell line 
      model. A549 cells were treated with either 600 AU/mL dialyzed D. pteronyssinus 
      immunotherapy extract (eDp) or Ctl media (Ctl) for 24 hours. Cells and 
      supernatants were collected, washed, and treated with Annexin V-FITC Apoptosis 
      Detection Kit II (BD Pharmingen, La Jolla, CA) and then analyzed by flow 
      cytometry. TGF-beta1 secretion was determined by ELISA using cell culture 
      supernatants. RESULTS: The eDp group showed a fourfold increase in early 
      apoptotic cells with a twofold increase in late apoptotic cells versus the Ctl 
      group, along with a 1.65-fold increase of TGF-beta1. CONCLUSION: eDp induced 
      viable A549 cells to undergo apoptosis determined by flow cytometry analysis with 
      a significant increase in TGF-beta1 secretion compared with Ctl.
FAU - Frisella, Patrick D
AU  - Frisella PD
AD  - University of New York Downstate Medical Center, Center for Allergy and Asthma 
      Research, Brooklyn, New York, USA. mfrieri@numc.edu
FAU - Silverberg, Jonathan
AU  - Silverberg J
FAU - Joks, Rauno
AU  - Joks R
FAU - Frieri, Marianne
AU  - Frieri M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Cell Extracts)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Airway Remodeling
MH  - Animals
MH  - Antigens, Dermatophagoides/*administration & dosage/immunology
MH  - Apoptosis
MH  - Cell Extracts/*administration & dosage/immunology
MH  - Cell Line, Tumor
MH  - Dermatophagoides pteronyssinus
MH  - Epithelial Cells/*metabolism/pathology
MH  - Epithelial-Mesenchymal Transition
MH  - Humans
MH  - *Immunotherapy
MH  - Pulmonary Alveoli/pathology
MH  - Rhinitis, Allergic, Perennial/*immunology
MH  - Sinusitis
MH  - Transforming Growth Factor beta/genetics/*metabolism
EDAT- 2011/08/09 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/08/09 06:00
PHST- 2011/08/09 06:00 [entrez]
PHST- 2011/08/09 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.2500/ajra.2011.25.3629 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2011 Jul-Aug;25(4):231-5. doi: 10.2500/ajra.2011.25.3629.