PMID- 21820435
OWN - NLM
STAT- MEDLINE
DCOM- 20111027
LR  - 20140115
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 585
IP  - 17
DP  - 2011 Sep 2
TI  - Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with 
      guanylate cyclase activity in vitro.
PG  - 2693-7
LID - 10.1016/j.febslet.2011.07.023 [doi]
AB  - While there is evidence of nitric oxide (NO)-dependent signalling via the second 
      messenger cyclic guanosine 3',5'-monophosphate (cGMP) in plants, guanylate 
      cyclases (GCs), enzymes that catalyse the formation of cGMP from guanosine 
      5'-triphosphate (GTP) have until recently remained elusive and none of the 
      candidates identified to-date are NO-dependent. Using both a GC and heme-binding 
      domain specific (H-NOX) search motif, we have identified an Arabidopsis flavin 
      monooxygenase (At1g62580) and shown electrochemically that it binds NO, has a 
      higher affinity for NO than for O(2) and that this molecule can generate cGMP 
      from GTP in vitro in an NO-dependent manner.
CI  - Copyright (c) 2011 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Mulaudzi, Takalani
AU  - Mulaudzi T
AD  - Department of Biotechnology, University of the Western Cape, Bellville, South 
      Africa.
FAU - Ludidi, Ndiko
AU  - Ludidi N
FAU - Ruzvidzo, Oziniel
AU  - Ruzvidzo O
FAU - Morse, Monique
AU  - Morse M
FAU - Hendricks, Nicolette
AU  - Hendricks N
FAU - Iwuoha, Emmanuel
AU  - Iwuoha E
FAU - Gehring, Chris
AU  - Gehring C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110731
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Arabidopsis Proteins)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.- (NOGC1 protein, Arabidopsis)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/enzymology/genetics/*metabolism
MH  - Arabidopsis Proteins/chemistry/genetics/*metabolism
MH  - Electrochemistry
MH  - Guanylate Cyclase/chemistry/*metabolism
MH  - Mixed Function Oxygenases/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Nitric Oxide/*metabolism
MH  - Oxygen/metabolism
EDAT- 2011/08/09 06:00
MHDA- 2011/10/28 06:00
CRDT- 2011/08/09 06:00
PHST- 2011/06/03 00:00 [received]
PHST- 2011/07/19 00:00 [revised]
PHST- 2011/07/19 00:00 [accepted]
PHST- 2011/08/09 06:00 [entrez]
PHST- 2011/08/09 06:00 [pubmed]
PHST- 2011/10/28 06:00 [medline]
AID - S0014-5793(11)00552-7 [pii]
AID - 10.1016/j.febslet.2011.07.023 [doi]
PST - ppublish
SO  - FEBS Lett. 2011 Sep 2;585(17):2693-7. doi: 10.1016/j.febslet.2011.07.023. Epub 
      2011 Jul 31.