PMID- 21821730
OWN - NLM
STAT- MEDLINE
DCOM- 20111222
LR  - 20200930
IS  - 1522-1504 (Electronic)
IS  - 1040-0605 (Linking)
VI  - 301
IP  - 5
DP  - 2011 Nov
TI  - Improved bioavailability of epoxyeicosatrienoic acids reduces TP-receptor 
      agonist-induced tension in human bronchi.
PG  - L675-82
LID - 10.1152/ajplung.00427.2010 [doi]
AB  - Epoxyeicosatrienoic acid (EET) and thromboxane A(2) are arachidonic acid 
      derivatives. The former has initially been defined as an epithelium-derived 
      hyperpolarizing factor displaying broncho-relaxing and anti-inflammatory 
      properties, as recently demonstrated, whereas thromboxane A(2) induces vaso- and 
      bronchoconstriction upon binding to thromboxane-prostanoid (TP)-receptor. EETs, 
      however, are quickly degraded by the soluble epoxide hydrolase (sEH) into 
      inactive diol compounds. The aim of this study was to investigate the effects of 
      14,15-EET on TP-receptor activation in human bronchi. Tension measurements 
      performed on native bronchi from various species, acutely treated with increasing 
      14,15-EET concentrations, revealed specific and concentration-dependent 
      relationships as well as a decrease in the tension induced by 30 nM U-46619, used 
      as a synthetic TP-receptor agonist. Interestingly, acute treatments with 3 muM 
      N-(methylsulfonyl)-2-(2-propynyloxy)-benzenehexanamide, an epoxygenase inhibitor, 
      which minimizes endogenous production of EET, resulted in an increased reactivity 
      to U-46619. Furthermore, we demonstrated that chronic treatments with 
      trans-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid (t-AUCB), a sEH 
      inhibitor, reduced human bronchi reactivity to U-46619. During our tension 
      measurements, we also observed that human bronchi generated small-amplitude 
      contractions; these spontaneous activities were reduced upon acute 14,15-EET 
      treatments in the presence of t-AUCB. Altogether, these data demonstrate that 
      endogenous and exogenous 14,15-EET could interfere with the activation of 
      TP-receptors as well as with spontaneous oscillations in human airway smooth 
      muscle tissues.
FAU - Senouvo, Farid Yannick
AU  - Senouvo FY
AD  - Le Bilarium, Department of Physiology and Biophysics, Faculty of Medicine and 
      Health Sciences, Universite de Sherbrooke, Sherbrooke, Quebec, Canada.
FAU - Tabet, Yacine
AU  - Tabet Y
FAU - Morin, Caroline
AU  - Morin C
FAU - Albadine, Roula
AU  - Albadine R
FAU - Sirois, Chantal
AU  - Sirois C
FAU - Rousseau, Eric
AU  - Rousseau E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110805
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (4-(4-(3-adamantan-1-ylureido)cyclohexyloxy)benzoic acid)
RN  - 0 (Benzoates)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Receptors, Prostaglandin E)
RN  - 0 (Receptors, Thromboxane)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 57576-52-0 (Thromboxane A2)
RN  - 76898-47-0 (15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid)
RN  - 81276-03-1 (14,15-epoxy-5,8,11-eicosatrienoic acid)
RN  - 8W8T17847W (Urea)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - FC398RK06S (8,11,14-Eicosatrienoic Acid)
SB  - IM
MH  - 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid/pharmacology
MH  - 8,11,14-Eicosatrienoic Acid/*analogs & derivatives/pharmacology
MH  - Animals
MH  - Arachidonic Acid/metabolism
MH  - Benzoates/pharmacology
MH  - Blotting, Western
MH  - Bronchi/cytology/*drug effects/physiology
MH  - Cells, Cultured
MH  - Eicosapentaenoic Acid/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epoxide Hydrolases/antagonists & inhibitors/metabolism
MH  - Epoxy Compounds
MH  - Fluorescent Antibody Technique
MH  - Guinea Pigs
MH  - Humans
MH  - Mice
MH  - Muscle Tonus/*drug effects
MH  - Muscle, Smooth/cytology/*drug effects/physiology
MH  - Myocytes, Smooth Muscle/cytology/*drug effects/physiology
MH  - Rats
MH  - Receptors, Prostaglandin E/agonists/antagonists & inhibitors/*metabolism
MH  - Receptors, Thromboxane/agonists/antagonists & inhibitors/*metabolism
MH  - *Signal Transduction
MH  - Thromboxane A2/metabolism
MH  - Urea/analogs & derivatives/pharmacology
EDAT- 2011/08/09 06:00
MHDA- 2011/12/23 06:00
CRDT- 2011/08/09 06:00
PHST- 2011/08/09 06:00 [entrez]
PHST- 2011/08/09 06:00 [pubmed]
PHST- 2011/12/23 06:00 [medline]
AID - ajplung.00427.2010 [pii]
AID - 10.1152/ajplung.00427.2010 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2011 Nov;301(5):L675-82. doi: 
      10.1152/ajplung.00427.2010. Epub 2011 Aug 5.