PMID- 21821816
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20120104
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 90
IP  - 1
DP  - 2012 Jan
TI  - Transcript abundance of hormone receptors, mammalian target of rapamycin 
      pathway-related kinases, insulin-like growth factor I, and milk proteins in 
      porcine mammary tissue.
PG  - 221-30
LID - 10.2527/jas.2011-4179 [doi]
AB  - Prolactin, glucocorticoids, and insulin are commonly used to induce milk protein 
      synthesis in bovine mammary cell cultures. In addition, administration of GH 
      increases milk yield in dairy cows, likely via the mammalian target of rapamycin 
      (mTOR) pathway and IGF-I synthesis. As such, the hypothesis of this study was 
      that mRNA abundance of hormone receptors, mammalian target of mTOR 
      pathway-related kinases, IGF-I, and milk protein-encoding genes increases in the 
      porcine mammary gland in response to greater lactation demand. Selected genes 
      included those encoding for receptors of GH (GHR), insulin (INSR), glucocorticoid 
      (NR3C1), prolactin (PRLR), IGF-I (IGF-I), mTOR (FRAP1), and p70S6 kinases 
      (RPS6KB1), and the milk proteins alpha-lactalbumin (LALBA) and beta-casein (CSN2). 
      Mammary tissue was biopsied from 4 sows on d 110 of gestation (prepartum), d 5 
      and 17 of lactation, and d 5 after weaning (postweaning), and gene expression was 
      quantified by reverse-transcription quantitative PCR. Compared with prepartum, d 
      5 of lactation increased (P < 0.001) NR3C1, tended to increase (P = 0.06) GHR, 
      and decreased (P < 0.001) PRLR mRNA abundance. Compared with d 5 of lactation, d 
      17 of lactation increased PRLR (P < 0.001) and decreased GHR (P < 0.01). 
      Expression of INR and FRAP1 did not differ when comparing either prepartum or d 
      17 of lactation with d 5 of lactation. Compared with d 17 of lactation, 
      postweaning decreased (P < 0.001) PRLR, did not affect INSR, and increased both 
      IGF-I and GHR (P < 0.05) mRNA abundance. From prepartum to d 17 of lactation, 
      NR3C1 mRNA abundance was positively correlated with CSN2 (r = 0.85; P < 0.001) 
      and LALBA mRNA abundance (r = 0.79; P = 0.002), whereas mRNA abundance of GHR 
      tended to be positively correlated with that of IGF-I (r = 0.46; P = 0.06). In 
      conclusion, expression of the genes NR3C1, PRLR, GHR, and IGF-I changed in the 
      porcine mammary gland during the prepartum to postweaning periods, but only NR3C1 
      mRNA abundance was positively correlated with expression of CSN2 and LALBA.
FAU - Manjarin, R
AU  - Manjarin R
AD  - Department of Animal Science, Michigan State University, East Lansing, MI 48824, 
      USA.
FAU - Steibel, J P
AU  - Steibel JP
FAU - Kirkwood, R N
AU  - Kirkwood RN
FAU - Taylor, N P
AU  - Taylor NP
FAU - Trottier, N L
AU  - Trottier NL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110805
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Milk Proteins)
RN  - 0 (Peptide Hormones)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Regulation
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Lactation
MH  - Mammary Glands, Animal/*metabolism
MH  - Milk Proteins/genetics/*metabolism
MH  - Peptide Hormones/genetics/*metabolism
MH  - Peripartum Period
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sus scrofa/genetics/*metabolism
MH  - Weaning
EDAT- 2011/08/09 06:00
MHDA- 2012/05/05 06:00
CRDT- 2011/08/09 06:00
PHST- 2011/08/09 06:00 [entrez]
PHST- 2011/08/09 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
AID - jas.2011-4179 [pii]
AID - 10.2527/jas.2011-4179 [doi]
PST - ppublish
SO  - J Anim Sci. 2012 Jan;90(1):221-30. doi: 10.2527/jas.2011-4179. Epub 2011 Aug 5.