PMID- 2182228
OWN - NLM
STAT- MEDLINE
DCOM- 19900518
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 55
IP  - 2
DP  - 1990 May
TI  - Leukocyte immunophenotyping by flow cytometry in a multisite study: 
      standardization, quality control, and normal values in the Transfusion Safety 
      Study. The Transfusion Safety Study Group.
PG  - 187-220
AB  - The Transfusion Safety Study (TSS) is a multicenter, cooperative investigation of 
      factors that may determine the occurrence and modify the expression of 
      transfusion-transmitted infections. A flow cytometry laboratory was established 
      in each of the six participating centers in order to avoid alterations in cell 
      phenotypes which may be caused by shipping delays, temperature changes, and 
      handling. As a consequence, in order to assure compatibility of results, 
      stringent standardization, quality control, and proficiency testing procedures 
      were developed. This paper documents (i) the effect of time from phlebotomy to 
      specimen staining and then to analysis for the antibodies used in the study; (ii) 
      the effects of variations in light scatter cursor location for certain 
      antibodies; (iii) a quality control program and data management and analysis 
      system, each specifically designed for the study; and (iv) presents extensive 
      data on age- and sex-related reference (normal) ranges for the several individual 
      and paired monoclonal antibodies used in the study. Problems encountered, 
      including obtaining reliable absolute lymphocyte counts, interference by 
      nucleated erythrocytes, and sources of variability in results, are discussed. 
      This study is meant to serve as a reference for future TSS publications.
FAU - Parker, J W
AU  - Parker JW
AD  - University of Southern California School of Medicine, Los Angeles 90032.
FAU - Adelsberg, B
AU  - Adelsberg B
FAU - Azen, S P
AU  - Azen SP
FAU - Boone, D
AU  - Boone D
FAU - Fletcher, M A
AU  - Fletcher MA
FAU - Gjerset, G F
AU  - Gjerset GF
FAU - Hassett, J
AU  - Hassett J
FAU - Kaplan, J
AU  - Kaplan J
FAU - Niland, J C
AU  - Niland JC
FAU - Odom-Maryon, T
AU  - Odom-Maryon T
AU  - et al.
LA  - eng
GR  - N01-HB-7003/HB/NHLBI NIH HHS/United States
GR  - N01-HB-9-7074/HB/NHLBI NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
SB  - IM
MH  - Blood Transfusion/standards
MH  - Data Interpretation, Statistical
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Leukocytes/*cytology/immunology
MH  - Multicenter Studies as Topic
MH  - Phenotype
MH  - Quality Control
MH  - Reference Values
MH  - Time Factors
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
AID - 10.1016/0090-1229(90)90097-a [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1990 May;55(2):187-220. doi: 
      10.1016/0090-1229(90)90097-a.