PMID- 21822548
OWN - NLM
STAT- MEDLINE
DCOM- 20120229
LR  - 20220408
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 123
IP  - 8
DP  - 2011 Dec
TI  - Multiple alleles for resistance and susceptibility modulate the defense response 
      in the interaction of tetraploid potato (Solanum tuberosum) with Synchytrium 
      endobioticum pathotypes 1, 2, 6 and 18.
PG  - 1281-92
LID - 10.1007/s00122-011-1666-9 [doi]
AB  - The obligate biotrophic, soil-borne fungus Synchytrium endobioticum causes wart 
      disease of potato (Solanum tuberosum), which is a serious problem for crop 
      production in countries with moderate climates. S. endobioticum induces 
      hypertrophic cell divisions in plant host tissues leading to the formation of 
      tumor-like structures. Potato wart is a quarantine disease and chemical control 
      is not possible. From 38 S. endobioticum pathotypes occurring in Europe, 
      pathotypes 1, 2, 6 and 18 are the most relevant. Genetic resistance to wart is 
      available but only few current potato varieties are resistant to all four 
      pathotypes. The phenotypic evaluation of wart resistance is laborious, 
      time-consuming and sometimes ambiguous, which makes breeding for resistance 
      difficult. Molecular markers diagnostic for genes for resistance to S. 
      endobioticum pathotypes 1, 2, 6 and 18 would greatly facilitate the selection of 
      new, resistant cultivars. Two tetraploid half-sib families (266 individuals) 
      segregating for resistance to S. endobioticum pathotypes 1, 2, 6 and 18 were 
      produced by crossing a resistant genotype with two different susceptible ones. 
      The families were scored for five different wart resistance phenotypes. The 
      distribution of mean resistance scores was quantitative in both families. 
      Resistance to pathotypes 2, 6 and 18 was correlated and independent from 
      resistance to pathotype 1. DNA pools were constructed from the most resistant and 
      most susceptible individuals and screened with genome wide simple sequence repeat 
      (SSR), inverted simple sequence region (ISSR) and randomly amplified polymorphic 
      DNA (RAPD) markers. Bulked segregant analysis identified three SSR markers that 
      were linked to wart resistance loci (Sen). Sen1-XI on chromosome XI conferred 
      partial resistance to pathotype 1, Sen18-IX on chromosome IX to pathotype 18 and 
      Sen2/6/18-I on chromosome I to pathotypes 2,6 and 18. Additional genotyping with 
      191 single nucleotide polymorphism (SNP) markers confirmed the localization of 
      the Sen loci. Thirty-three SNP markers linked to the Sen loci permitted the 
      dissection of Sen alleles that increased or decreased resistance to wart. The 
      alleles were inherited from both the resistant and susceptible parents.
FAU - Ballvora, Agim
AU  - Ballvora A
AD  - Max-Planck-Institute for Plant Breeding Research, Cologne, Germany.
FAU - Flath, Kerstin
AU  - Flath K
FAU - Lubeck, Jens
AU  - Lubeck J
FAU - Strahwald, Josef
AU  - Strahwald J
FAU - Tacke, Eckhard
AU  - Tacke E
FAU - Hofferbert, Hans-Reinhard
AU  - Hofferbert HR
FAU - Gebhardt, Christiane
AU  - Gebhardt C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110806
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Alleles
MH  - Chromosomes, Plant/genetics
MH  - Chytridiomycota/*physiology
MH  - Disease Resistance/*genetics
MH  - Genetic Loci/genetics
MH  - Genetic Markers
MH  - Host-Pathogen Interactions/*genetics
MH  - Microsatellite Repeats/genetics
MH  - Phenotype
MH  - Plant Diseases/genetics/immunology/*microbiology
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Principal Component Analysis
MH  - Solanum tuberosum/genetics/*immunology/microbiology
MH  - *Tetraploidy
PMC - PMC3214262
EDAT- 2011/08/09 06:00
MHDA- 2012/03/01 06:00
PMCR- 2011/08/06
CRDT- 2011/08/09 06:00
PHST- 2011/03/08 00:00 [received]
PHST- 2011/07/16 00:00 [accepted]
PHST- 2011/08/09 06:00 [entrez]
PHST- 2011/08/09 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
PHST- 2011/08/06 00:00 [pmc-release]
AID - 1666 [pii]
AID - 10.1007/s00122-011-1666-9 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2011 Dec;123(8):1281-92. doi: 10.1007/s00122-011-1666-9. Epub 
      2011 Aug 6.