PMID- 21823428
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 31
IP  - 6
DP  - 2011 Jun
TI  - [Effects of scutellaria stem-leaf total flavonoids on cardiocyte apoptosis 
      induced by hypoxia/reoxygenation].
PG  - 803-6
AB  - OBJECTIVE: To study the effect of Scutellaria baicalensis stem-leaf total 
      flavonoids (SSTF) on cardiocyte apoptosis of neonatal rats induced by 
      hypoxia/reoxygenation and its action of mechanism. METHODS Sixty one to two days 
      old rats, male or female, were selected. Hypoxia/reoxygenation injured model was 
      established in cultured cardiocytes of neonate rats. The cultured neonatal rat 
      cardiocytes were divided into 5 groups, i.e., the normal control group, the 
      hypoxia/reoxygenation injury group (as the model group, cultured cardiocytes were 
      exposed to hypoxia 2 h and subsequently reoxygenated for 4 h), the 
      hypoxia/reoxygenation injury plus 50 mg/L SSTF group (as the low dose SSTF 
      group), the hypoxia/reoxygenation plus 100 mg/L SSTF group (as the middle dose 
      SSTF group), and the hypoxia/reoxygenation plus 200 mg/L SSTF group (as the high 
      dose SSTF group). The cell viability was detected by methyl thiazolyl tetrazolium 
      (MTT) colorimetry. The apoptosis of cardiocytes was determined by terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and the 
      apoptosis rate calculated. The Bcl-2 and Bax protein expressions were determined 
      by immunohistochemistry. RESULTS: Compared with the normal control group, the 
      cell viability, Bcl-2 protein contents and Bcl-2/Bax decreased, the apoptosis 
      rate and Bax protein contents increased in the model group (all P<0.01). Compared 
      with the model group, the cell viability, Bcl-2 protein contents and Bcl-2/Bax 
      increased, while the apoptosis rate and Bax protein contents decreased in each 
      SSTF treated group (P<0.05, P<0.01). Compared with the low dose SSTF group, 
      significant difference existed in each index of the high dose SSTF group (all 
      P<0.05). CONCLUSIONS: SSTF had protection on hypoxia/reoxygenation induced 
      cardiocyte apoptosis. Its protective mechanism might be correlated with its 
      up-regulation of the expression of Bcl-2 protein ahd down-regulation of the 
      expression of Bax protein.
FAU - Zhou, Xiao-hui
AU  - Zhou XH
AD  - Department of Biochemistry, Chengde Medical College, Hebei 067000. 
      zxh5055@sina.com
FAU - Gong, Ming-yu
AU  - Gong MY
FAU - Yang, He-mei
AU  - Yang HM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Bax protein, rat)
RN  - 0 (Flavonoids)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Flavonoids/*pharmacology
MH  - Male
MH  - Myocytes, Cardiac/*drug effects/metabolism
MH  - Plant Stems/chemistry
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Scutellaria/chemistry
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2011/08/10 06:00
MHDA- 2013/08/02 06:00
CRDT- 2011/08/10 06:00
PHST- 2011/08/10 06:00 [entrez]
PHST- 2011/08/10 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Jun;31(6):803-6.