PMID- 21829524
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 7
DP  - 2011
TI  - Adiponectin upregulates prolyl-4-hydroxylase alpha1 expression in interleukin 
      6-stimulated human aortic smooth muscle cells by regulating ERK 1/2 and Sp1.
PG  - e22819
LID - 10.1371/journal.pone.0022819 [doi]
LID - e22819
AB  - Adiponectin is an anti-atherogenic adipokine that inhibits the development of 
      plaque by mechanisms that are not completely understood. Extracellular matrix 
      (ECM) may have a role in the pathogenesis of atherosclerosis. We explored the 
      effect and mechanisms of adiponectin on the synthesis of prolyl-4-hydroxylase 
      (P4H) in interleukin 6 (IL-6)-stimulated human aortic smooth muscle cells 
      (HASMCs). P4Halpha1 mRNA level was quantified by RT-PCR, the protein levels of 
      phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and P4Halpha1 were 
      quantified by western blot analysis, and activation of specific protein 1 (Sp1) 
      was determined by electrophoretic mobility shift assay and subcellular 
      localization of Sp1 by immunofluorescence analysis. Adiponectin significantly 
      increased P4Halpha1 mRNA and protein levels in IL-6-stimulated HASMCs in a dose- and 
      time-dependent manner. As well, ERK1/2 and Sp1 played a crucial role in the 
      effect of adiponectin upregulating P4Halpha1 expression in IL-6-stimulated HASMCs. 
      Adiponectin abrogated the effects of IL-6 on collagen III level, which may 
      indicate that P4Halpha1 is essential for folding the procollagen polypeptide chains 
      into stabilized collagen. Adiponectin attenuates IL-6-inhibited P4Halpha1 synthesis 
      and stabilizes collagen formation in HASMCs through a Sp1-ERK1/2-P4Halpha1-dependent 
      pathway.
FAU - Li, Li
AU  - Li L
AD  - The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese 
      Ministry of Education, Shandong University Qilu Hospital, Jinan, Shandong, China.
FAU - Zhang, Ke
AU  - Zhang K
FAU - Cai, Xiao-Jun
AU  - Cai XJ
FAU - Feng, Min
AU  - Feng M
FAU - Zhang, Yun
AU  - Zhang Y
FAU - Zhang, Mei
AU  - Zhang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110729
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Adiponectin)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sp1 Transcription Factor)
RN  - EC 1.14.11.2 (P4HA1 protein, human)
RN  - EC 1.14.11.2 (Procollagen-Proline Dioxygenase)
RN  - EC 2.7.11.24 (MAPK1 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Adiponectin/*pharmacology
MH  - Aorta/cytology/drug effects/*metabolism
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Electrophoretic Mobility Shift Assay
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-6/*pharmacology
MH  - Mitogen-Activated Protein Kinase 1/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/genetics/*metabolism
MH  - Muscle, Smooth, Vascular/cytology/drug effects/*metabolism
MH  - Phosphorylation
MH  - Procollagen-Proline Dioxygenase/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Sp1 Transcription Factor/genetics/*metabolism
PMC - PMC3146504
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/08/11 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/29
CRDT- 2011/08/11 06:00
PHST- 2011/04/14 00:00 [received]
PHST- 2011/06/29 00:00 [accepted]
PHST- 2011/08/11 06:00 [entrez]
PHST- 2011/08/11 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/29 00:00 [pmc-release]
AID - PONE-D-11-06807 [pii]
AID - 10.1371/journal.pone.0022819 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(7):e22819. doi: 10.1371/journal.pone.0022819. Epub 2011 Jul 29.