PMID- 21830292
OWN - NLM
STAT- MEDLINE
DCOM- 20120109
LR  - 20211020
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Print)
IS  - 1439-4227 (Linking)
VI  - 12
IP  - 14
DP  - 2011 Sep 19
TI  - Isolation, amino acid sequence and biological activities of novel long-chain 
      polyamine-associated peptide toxins from the sponge Axinyssa aculeata.
PG  - 2191-200
LID - 10.1002/cbic.201100329 [doi]
AB  - A novel family of functionalized peptide toxins, aculeines (ACUs), was isolated 
      from the marine sponge Axinyssa aculeate. ACUs are polypeptides with N-terminal 
      residues that are modified by the addition of long-chain polyamines (LCPA). 
      Aculeines were present in the sponge extract as a complex mixture with differing 
      polyamine chain lengths and peptide structures. ACU-A and B, which were purified 
      in this study, share a common polypeptide chain but differ in their N-terminal 
      residue modifications. The amino acid sequence of the polypeptide portion of 
      ACU-A and B was deduced from 3' and 5' RACE, and supported by Edman degradation 
      and mass spectral analysis of peptide fragments. ACU induced convulsions upon 
      intracerebroventricular (i.c.v.) injection in mice, and disrupted neuronal 
      membrane integrity in electrophysiological assays. ACU also lysed erythrocytes 
      with a potency that differed between animal species. Here we describe the 
      isolation, amino acid sequence, and biological activity of this new group of 
      cytotoxic sponge peptides.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Matsunaga, Satoko
AU  - Matsunaga S
AD  - Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan.
FAU - Jimbo, Mitsuru
AU  - Jimbo M
FAU - Gill, Martin B
AU  - Gill MB
FAU - Wyhe, L Leanne Lash-Van
AU  - Wyhe LL
FAU - Murata, Michio
AU  - Murata M
FAU - Nonomura, Ken'ichi
AU  - Nonomura K
FAU - Swanson, Geoffrey T
AU  - Swanson GT
FAU - Sakai, Ryuichi
AU  - Sakai R
LA  - eng
GR  - P30 CA060553/CA/NCI NIH HHS/United States
GR  - R01 NS044322/NS/NINDS NIH HHS/United States
GR  - R01 NS044322-10/NS/NINDS NIH HHS/United States
GR  - R01 NS44322/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110809
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (Convulsants)
RN  - 0 (Peptides)
RN  - 0 (Polyamines)
RN  - 0 (Toxins, Biological)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Membrane Permeability
MH  - Cloning, Molecular
MH  - Convulsants/chemistry/isolation & purification/metabolism/toxicity
MH  - HEK293 Cells
MH  - Hemolysis/drug effects
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neurons/cytology/drug effects
MH  - Peptide Mapping
MH  - Peptides/*chemistry/*isolation & purification/metabolism/toxicity
MH  - Polyamines/*chemistry/*metabolism
MH  - Porifera/*chemistry/genetics
MH  - Sequence Analysis, DNA
MH  - Toxins, Biological/*chemistry/*isolation & purification/metabolism/toxicity
PMC - PMC3533443
MID - NIHMS341091
EDAT- 2011/08/11 06:00
MHDA- 2012/01/10 06:00
PMCR- 2012/12/31
CRDT- 2011/08/11 06:00
PHST- 2011/05/24 00:00 [received]
PHST- 2011/08/11 06:00 [entrez]
PHST- 2011/08/11 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
PHST- 2012/12/31 00:00 [pmc-release]
AID - 10.1002/cbic.201100329 [doi]
PST - ppublish
SO  - Chembiochem. 2011 Sep 19;12(14):2191-200. doi: 10.1002/cbic.201100329. Epub 2011 
      Aug 9.