PMID- 21835647
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20211020
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 35
IP  - 3
DP  - 2011 Sep 23
TI  - The signaling adaptor Eps8 is an essential actin capping protein for dendritic 
      cell migration.
PG  - 388-99
LID - 10.1016/j.immuni.2011.07.007 [doi]
AB  - Dendritic cells (DCs) flexibly adapt to different microenvironments by using 
      diverse migration strategies that are ultimately dependent on the dynamics and 
      structural organization of the actin cytoskeleton. Here, we have shown that DCs 
      require the actin capping activity of the signaling adaptor Eps8 to polarize and 
      to form elongated migratory protrusions. DCs from Eps8-deficient mice are 
      impaired in directional and chemotactic migration in 3D in vitro and are delayed 
      in reaching the draining lymph node (DLN) in vivo after inflammatory challenge. 
      Hence, Eps8-deficient mice are unable to mount a contact hypersensitivity 
      response. We have also shown that the DC migratory defect is cell autonomous and 
      that Eps8 is required for the proper architectural organization of the actin 
      meshwork and dynamics of cell protrusions. Yet, Eps8 is not necessary for antigen 
      uptake, processing, and presentation. Thus, we have identified Eps8 as a unique 
      actin capping protein specifically required for DC migration.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Frittoli, Emanuela
AU  - Frittoli E
AD  - IFOM, FIRC Institute of Molecular Oncology Foundation at IFOM-IEO Campus, Via 
      Adamello 16, 20139 Milan, Italy.
FAU - Matteoli, Gianluca
AU  - Matteoli G
FAU - Palamidessi, Andrea
AU  - Palamidessi A
FAU - Mazzini, Elisa
AU  - Mazzini E
FAU - Maddaluno, Luigi
AU  - Maddaluno L
FAU - Disanza, Andrea
AU  - Disanza A
FAU - Yang, Changsong
AU  - Yang C
FAU - Svitkina, Tatyana
AU  - Svitkina T
FAU - Rescigno, Maria
AU  - Rescigno M
FAU - Scita, Giorgio
AU  - Scita G
LA  - eng
GR  - R01 GM070898/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110811
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Abi1 protein, mouse)
RN  - 0 (Actin Capping Proteins)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cytoskeletal Proteins)
SB  - IM
MH  - Actin Capping Proteins/*immunology
MH  - Adaptor Proteins, Signal Transducing/deficiency/genetics/*immunology
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Movement/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytoskeletal Proteins/deficiency/genetics/*immunology
MH  - Dendritic Cells/*immunology
MH  - Dermatitis, Contact/immunology
MH  - Flow Cytometry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - *Signal Transduction
MH  - T-Lymphocytes/immunology
PMC - PMC3424277
MID - NIHMS394262
EDAT- 2011/08/13 06:00
MHDA- 2011/11/16 06:00
PMCR- 2012/09/23
CRDT- 2011/08/13 06:00
PHST- 2011/01/26 00:00 [received]
PHST- 2011/05/16 00:00 [revised]
PHST- 2011/07/08 00:00 [accepted]
PHST- 2011/08/13 06:00 [entrez]
PHST- 2011/08/13 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PHST- 2012/09/23 00:00 [pmc-release]
AID - S1074-7613(11)00275-5 [pii]
AID - 10.1016/j.immuni.2011.07.007 [doi]
PST - ppublish
SO  - Immunity. 2011 Sep 23;35(3):388-99. doi: 10.1016/j.immuni.2011.07.007. Epub 2011 
      Aug 11.