PMID- 21839264
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20171116
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 43
IP  - 6
DP  - 2011 Jul-Aug
TI  - Delivery of alloantigens via apoptotic cells generates dendritic cells with an 
      immature tolerogenic phenotype.
PG  - 2325-33
LID - 10.1016/j.transproceed.2011.06.007 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells able 
      to induce immunity or tolerance. The interactions of immature DCs with naive T 
      lymphocytes induce peripheral tolerance through mechanisms that include anergy or 
      deletion of lymphocytes or the generation of regulatory T cells. Because of the 
      central role of DCs in the immune response, they are potential targets for the 
      induction of experimental tolerance. Thus, the generation of immature 
      (tolerogenic) DCs able to capture and present alloantigens to T cells represents 
      an important aim in our efforts to achieve better transplant acceptance. METHODS: 
      In this work, we generated immature DCs by using vitamin D(3) (VD3) during the 
      process of DC differentiation. RESULTS: The VD3-DCs showed an immature phenotype 
      characterized by a low expression of major histocompatibility complex antigens of 
      class II, CD86, and CD80 molecules and the secretion of a tolerogenic cytokine 
      pattern. Furthermore, we showed that VD3-DCs phagocytose apoptotic allogeneic 
      cells efficiently without inducing DC maturation or activation. Most important, 
      our experiments demonstrated that mice treated with VD3 produce immature DCs in 
      vivo, and that DCs from VD3-treated mice immunized with allogeneic apoptotic 
      cells maintained their tolerogenic phenotype. CONCLUSION: Our results show that 
      allogeneic apoptotic cells in combination with VD3 generate DCs with tolerogenic 
      characteristics that could be used to induce tolerance towards alloantigens.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Gleisner, M A
AU  - Gleisner MA
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Rosemblatt, M
AU  - Rosemblatt M
FAU - Fierro, J A
AU  - Fierro JA
FAU - Bono, M R
AU  - Bono MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (B7-1 Antigen)
RN  - 0 (CD68 protein, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Isoantigens)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
RN  - 1C6V77QF41 (Cholecalciferol)
SB  - IM
MH  - Animals
MH  - Antigens, CD/immunology
MH  - Antigens, Differentiation, Myelomonocytic/immunology
MH  - *Apoptosis
MH  - B7-1 Antigen/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cholecalciferol/pharmacology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/drug effects/*immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - *Immune Tolerance
MH  - Immunophenotyping
MH  - Isoantigens/*immunology
MH  - Male
MH  - Melanoma, Experimental/genetics/immunology
MH  - Membrane Proteins/genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Necrosis
MH  - *Phagocytosis
MH  - Phenotype
MH  - Thymocytes/*immunology/pathology/transplantation
MH  - Time Factors
MH  - Transfection
EDAT- 2011/08/16 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/08/16 06:00
PHST- 2011/08/16 06:00 [entrez]
PHST- 2011/08/16 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - S0041-1345(11)00815-3 [pii]
AID - 10.1016/j.transproceed.2011.06.007 [doi]
PST - ppublish
SO  - Transplant Proc. 2011 Jul-Aug;43(6):2325-33. doi: 
      10.1016/j.transproceed.2011.06.007.