PMID- 21839433
OWN - NLM
STAT- MEDLINE
DCOM- 20111129
LR  - 20191210
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 96
IP  - 4
DP  - 2011 Oct
TI  - Oxidative deoxyribonucleic acid damage in sperm has a negative impact on clinical 
      pregnancy rate in intrauterine insemination but not intracytoplasmic sperm 
      injection cycles.
PG  - 843-7
LID - 10.1016/j.fertnstert.2011.07.356 [doi]
AB  - OBJECTIVE: To determine the level of DNA damage, both fragmentation and 
      oxidative, in the sperm population used for intrauterine insemination (IUI) and 
      intracytoplasmic sperm injection (ICSI) and its impact on fertilization and 
      clinical pregnancy rates. DESIGN: A prospective clinical study. SETTING: A 
      tertiary care fertility clinic. PATIENT(S): Couples undergoing ICSI (n = 48) and 
      couples undergoing IUI cycles (n = 53). INTERVENTION(S): Assessment of both sperm 
      DNA fragmentation using the TUNEL assay and oxidative DNA damage using the 
      biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the samples prepared and used 
      for insemination. MAIN OUTCOME MEASURE(S): Achievement of a clinical pregnancy. 
      RESULT(S): Sperm DNA fragmentation and 8-OHdG were highly correlated (r = 0.55) 
      and 8-OHdG was significantly lower in those who achieved a clinical pregnancy 
      after IUI (8.9% vs. 20.2%). A threshold value of 11.5% 8-OHdG was identified as a 
      useful predictor of IUI success. No differences were found in sperm DNA 
      fragmentation or 8-OHdG between pregnant and nonpregnant couples in ICSI cycles. 
      CONCLUSION(S): 8-Hydroxy-2'-deoxyguanosine, a biomarker of oxidative DNA damage 
      highly correlated with sperm DNA fragmentation, in human sperm DNA has 
      significant value in predicting the chance of a clinical pregnancy after IUI but 
      not ICSI in assisted reproductive technology.
CI  - Copyright (c) 2011 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Thomson, Laura Kelly
AU  - Thomson LK
AD  - Fertility First, Hurstville, New South Wales, Australia. l.surmon@uws.edu.au
FAU - Zieschang, Julie-Anne
AU  - Zieschang JA
FAU - Clark, Anne Melton
AU  - Clark AM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110811
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - 9007-49-2 (DNA)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
CIN - J Urol. 2012 Aug;188(2):555-6. PMID: 22784757
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adult
MH  - DNA/chemistry/genetics/*metabolism
MH  - DNA Damage/*physiology
MH  - DNA Fragmentation
MH  - Deoxyguanosine/analogs & derivatives/analysis
MH  - Female
MH  - Humans
MH  - Insemination, Artificial, Homologous/*trends
MH  - Male
MH  - Oxidative Stress/*physiology
MH  - Pregnancy
MH  - Pregnancy Rate/*trends
MH  - Prospective Studies
MH  - Sperm Injections, Intracytoplasmic/*trends
MH  - Spermatozoa/*metabolism
EDAT- 2011/08/16 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/08/16 06:00
PHST- 2011/03/09 00:00 [received]
PHST- 2011/06/10 00:00 [revised]
PHST- 2011/07/07 00:00 [accepted]
PHST- 2011/08/16 06:00 [entrez]
PHST- 2011/08/16 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0015-0282(11)01453-1 [pii]
AID - 10.1016/j.fertnstert.2011.07.356 [doi]
PST - ppublish
SO  - Fertil Steril. 2011 Oct;96(4):843-7. doi: 10.1016/j.fertnstert.2011.07.356. Epub 
      2011 Aug 11.