PMID- 21839789
OWN - NLM
STAT- MEDLINE
DCOM- 20120117
LR  - 20110929
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 161
IP  - 2
DP  - 2011 Nov
TI  - Large scale parallel pyrosequencing technology: PRRSV strain VR-2332 nsp2 
      deletion mutant stability in swine.
PG  - 162-9
LID - 10.1016/j.virusres.2011.07.018 [doi]
AB  - Fifteen porcine reproductive and respiratory syndrome virus (PRRSV) isolate 
      genomes were derived simultaneously using 454 pyrosequencing technology. The 
      viral isolates sequenced were from a recent swine study, in which engineered Type 
      2 prototype PRRSV strain VR-2332 mutants, with 87, 184, 200, and 403 amino acid 
      deletions in the second hypervariable region of nsp2, were found to be stable in 
      the nsp2 coding region after in vivo infection (Faaberg et al., 2010). 
      Furthermore, 3 of 4 mutants achieved replication kinetics similar to wt virus by 
      study end. We hypothesized that other mutations elsewhere in the virus may have 
      contributed to their replication fitness in swine. To further assess the 
      stability of the engineered viruses, all sequenced genomes were compared and 
      contrasted. No specific mutations occurred in all nsp2 deletion mutant genomes 
      that were not also seen in the parent genome of Type 2 PRRSV strain VR-2332. 
      Second site (non-nsp2) deletions and/or insertions were not evident after 
      replication in swine. The number of point mutations seen increased slightly with 
      deletion size, but even the largest deletion (403 aa) had very few consensus 
      mutations. Thus, our findings provide further substantiation that the nsp2 
      deletion mutant genomes were genetically stable after in vivo passage.
CI  - Published by Elsevier B.V.
FAU - Guo, Baoqing
AU  - Guo B
AD  - Veterinary Diagnostic & Production Animal Medicine, Iowa State University, Ames, 
      IA, USA.
FAU - Vorwald, Ann C
AU  - Vorwald AC
FAU - Alt, David P
AU  - Alt DP
FAU - Lager, Kelly M
AU  - Lager KM
FAU - Bayles, Darrell O
AU  - Bayles DO
FAU - Faaberg, Kay S
AU  - Faaberg KS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110804
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Genome, Viral
MH  - *Genomic Instability
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/growth & 
      development/isolation & purification
MH  - Sequence Analysis, DNA
MH  - *Sequence Deletion
MH  - Sus scrofa
MH  - Swine
MH  - Viral Nonstructural Proteins/*genetics
EDAT- 2011/08/16 06:00
MHDA- 2012/01/18 06:00
CRDT- 2011/08/16 06:00
PHST- 2011/05/16 00:00 [received]
PHST- 2011/07/26 00:00 [revised]
PHST- 2011/07/27 00:00 [accepted]
PHST- 2011/08/16 06:00 [entrez]
PHST- 2011/08/16 06:00 [pubmed]
PHST- 2012/01/18 06:00 [medline]
AID - S0168-1702(11)00282-6 [pii]
AID - 10.1016/j.virusres.2011.07.018 [doi]
PST - ppublish
SO  - Virus Res. 2011 Nov;161(2):162-9. doi: 10.1016/j.virusres.2011.07.018. Epub 2011 
      Aug 4.