PMID- 21840035
OWN - NLM
STAT- MEDLINE
DCOM- 20120222
LR  - 20131121
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 85
IP  - 6
DP  - 2011 Oct
TI  - Developmental toxicity of cypermethrin in embryo-larval stages of zebrafish.
PG  - 1010-6
LID - 10.1016/j.chemosphere.2011.07.024 [doi]
AB  - Cypermethrin, a type II pyrethroid insecticide, is widely used throughout the 
      world in agriculture, forestry, horticulture and homes. Though the neurotoxicity 
      of cypermethrin has been thoroughly studied in adult rodents, little is so far 
      available regarding the developmental toxicity of cypermethrin to fish in early 
      life stages. To explore the potential developmental toxicity of cypermethrin, 4-h 
      post-fertilization (hpf) zebrafish embryos were exposed to various concentrations 
      of cypermethrin (0, 25, 50, 100, 200 and 400 mug L(-)(1)) until 96 h. Among a suite of 
      morphological abnormalities, the unique phenotype curvature was observed at 
      concentrations as low as 25 mug L(-)(1). Studies revealed that 400 mug L(-)(1) cypermethrin 
      significantly increased malondialdehyde production. In addition, activity of 
      antioxidative enzymes including superoxide dismutase and catalase were 
      significantly induced in zebrafish larvae in a concentration-dependent manner. To 
      further investigate the toxic effects of cypermethrin on fish, acridine orange 
      (AO) staining was performed at 400 mug L(-)(1) cypermethrin and the result showed 
      notable signs of apoptosis mainly in the nervous system. Cypermethrin also 
      down-regulated ogg1 and increased p53 gene expression as well as the caspase-3 
      activity. Our results demonstrate that cypermethrin was able to induce oxidative 
      stress and produce apoptosis through the involvement of caspases in zebrafish 
      embryos. In this study, we investigated the developmental toxicity of 
      cypermethrin using zebrafish embryos, which could be helpful in fully 
      understanding the potential mechanisms of cypermethrin exposure during 
      embryogenesis and also suggested that zebrafish could serve as an ideal model for 
      studying developmental toxicity of environmental contaminants.
CI  - Crown Copyright (c) 2011. Published by Elsevier Ltd. All rights reserved.
FAU - Shi, Xiangguo
AU  - Shi X
AD  - State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing 
      Medical University, Nanjing 210029, China.
FAU - Gu, Aihua
AU  - Gu A
FAU - Ji, Guixiang
AU  - Ji G
FAU - Li, Yuan
AU  - Li Y
FAU - Di, Jing
AU  - Di J
FAU - Jin, Jing
AU  - Jin J
FAU - Hu, Fan
AU  - Hu F
FAU - Long, Yan
AU  - Long Y
FAU - Xia, Yankai
AU  - Xia Y
FAU - Lu, Chuncheng
AU  - Lu C
FAU - Song, Ling
AU  - Song L
FAU - Wang, Shoulin
AU  - Wang S
FAU - Wang, Xinru
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110812
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Antioxidants)
RN  - 0 (Pesticide Residues)
RN  - 0 (Pyrethrins)
RN  - 0 (RNA, Messenger)
RN  - 059QF0KO0R (Water)
RN  - 1TR49121NP (cypermethrin)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Apoptosis/drug effects
MH  - Caspase 3/metabolism
MH  - DNA Repair/drug effects
MH  - Embryo, Nonmammalian/cytology/*drug effects/enzymology/metabolism
MH  - Endpoint Determination
MH  - Larva/cytology/drug effects/genetics/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Malondialdehyde/metabolism
MH  - Oxidation-Reduction
MH  - Pesticide Residues/*toxicity
MH  - Pyrethrins/*toxicity
MH  - RNA, Messenger/genetics/metabolism
MH  - Toxicity Tests, Acute/*methods
MH  - Transcriptome/drug effects
MH  - Water/chemistry
MH  - Zebrafish/*embryology/genetics/metabolism
EDAT- 2011/08/16 06:00
MHDA- 2012/02/23 06:00
CRDT- 2011/08/16 06:00
PHST- 2011/03/06 00:00 [received]
PHST- 2011/06/27 00:00 [revised]
PHST- 2011/07/18 00:00 [accepted]
PHST- 2011/08/16 06:00 [entrez]
PHST- 2011/08/16 06:00 [pubmed]
PHST- 2012/02/23 06:00 [medline]
AID - S0045-6535(11)00860-5 [pii]
AID - 10.1016/j.chemosphere.2011.07.024 [doi]
PST - ppublish
SO  - Chemosphere. 2011 Oct;85(6):1010-6. doi: 10.1016/j.chemosphere.2011.07.024. Epub 
      2011 Aug 12.