PMID- 21840420
OWN - NLM
STAT- MEDLINE
DCOM- 20120325
LR  - 20220316
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1811
IP  - 12
DP  - 2011 Dec
TI  - ChREBP expression in the liver, adipose tissue and differentiated preadipocytes 
      in human obesity.
PG  - 1194-200
LID - 10.1016/j.bbalip.2011.07.016 [doi]
AB  - ChREBP is an essential transcription factor for lipogenesis. Its physiological 
      role in adipose tissue has been studied only to a small extent and the control of 
      its expression remains unknown in human adipocytes. We have studied ChREBP mRNA 
      and protein expression levels in the liver and the omental (OM) and subcutaneous 
      (SC) adipose tissues from obese and lean subjects, as well as in human 
      differentiated preadipocytes. Liver and OM and SC adipose tissue biopsies were 
      obtained from lean and obese patients. Human preadipocytes were isolated from the 
      adipose tissues from obese patients and differentiated under adipogenic 
      conditions. ChREBP expression levels were quantified by RT-PCR and Western blot 
      analysis. We found opposing results in terms of ChREBP regulation in the liver 
      and adipose samples. ChREBP increased in the liver from obese compared to lean 
      subjects, whereas the expression decreased in both adipose tissues. The mRNAs of 
      other adipogenic markers were checked in these tissues. The pattern of FASN was 
      similar to the one for ChREBP, ADCY3 decreased in both adipose tissues from obese 
      patients, AP2 decreased only in OM adipose tissue of obese patients and ATGL did 
      not change. The levels of ChREBP mRNA and protein showed dramatic increases 
      during the differentiation of human OM and SC preadipocytes. In conclusion, 
      ChREBP expression has an opposite regulation in the liver and adipose tissue from 
      obese subjects which is compatible with the increased hepatic lipogenesis and 
      decreased adipocytic lipogenesis found in these patients. The dramatic increase 
      of ChREBP mRNA and protein levels during preadipocyte differentiation suggests a 
      role in adipogenesis.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Hurtado del Pozo, Carmen
AU  - Hurtado del Pozo C
AD  - Consejo Superior de Investigaciones Cientificas y Universidad Autonoma de Madrid, 
      Madrid, Spain.
FAU - Vesperinas-Garcia, Gregorio
AU  - Vesperinas-Garcia G
FAU - Rubio, Miguel-Angel
AU  - Rubio MA
FAU - Corripio-Sanchez, Ramon
AU  - Corripio-Sanchez R
FAU - Torres-Garcia, Antonio J
AU  - Torres-Garcia AJ
FAU - Obregon, Maria-Jesus
AU  - Obregon MJ
FAU - Calvo, Rosa Maria
AU  - Calvo RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110730
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (FABP4 protein, human)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (MLXIPL protein, human)
RN  - 0 (RNA, Messenger)
RN  - EC 2.3.1.85 (FASN protein, human)
RN  - EC 2.3.1.85 (Fatty Acid Synthase, Type I)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.1.1.3 (PNPLA2 protein, human)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - EC 4.6.1.1 (adenylate cyclase 3)
SB  - IM
MH  - Adenylyl Cyclases/genetics/metabolism
MH  - Adipocytes/cytology/*metabolism
MH  - *Adipogenesis
MH  - Adult
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics/*metabolism
MH  - Blotting, Western
MH  - Cell Differentiation
MH  - Fatty Acid Synthase, Type I/genetics/metabolism
MH  - Fatty Acid-Binding Proteins/genetics/metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Lipase/genetics/metabolism
MH  - Lipogenesis
MH  - Liver/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Obesity/*genetics/*metabolism/pathology
MH  - Omentum/cytology/*metabolism
MH  - Primary Cell Culture
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Subcutaneous Fat/cytology/*metabolism
EDAT- 2011/08/16 06:00
MHDA- 2012/03/27 06:00
CRDT- 2011/08/16 06:00
PHST- 2011/05/12 00:00 [received]
PHST- 2011/07/18 00:00 [revised]
PHST- 2011/07/24 00:00 [accepted]
PHST- 2011/08/16 06:00 [entrez]
PHST- 2011/08/16 06:00 [pubmed]
PHST- 2012/03/27 06:00 [medline]
AID - S1388-1981(11)00141-7 [pii]
AID - 10.1016/j.bbalip.2011.07.016 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2011 Dec;1811(12):1194-200. doi: 
      10.1016/j.bbalip.2011.07.016. Epub 2011 Jul 30.