PMID- 21842373
OWN - NLM
STAT- MEDLINE
DCOM- 20120828
LR  - 20211020
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 35
IP  - 2
DP  - 2012 Apr
TI  - Modulation of inflammatory processes by leaves extract from Clusia nemorosa both 
      in vitro and in vivo animal models.
PG  - 764-71
LID - 10.1007/s10753-011-9372-y [doi]
AB  - The present study was carried out to investigate the anti-inflammatory effect of 
      the hexane extract of the leaves from Clusia nemorosa G. Mey, called HECn, using 
      carrageenan-induced mice pleurisy and cotton pellet-induced mice granuloma. 
      Additionally, the ability of HECn to affect both neutrophil migration as 
      viability was investigated by use of the Boyden chamber assay and flow cytometry, 
      respectively. The HECn significantly inhibited exudation, total leukocytes and 
      neutrophils influx, as well as TNFalpha levels in carrageenan-induced pleurisy. 
      However, the extract not suppressed the granulomatous tissue formation in the 
      cotton pellet-induced granuloma test. Experiments performed in vitro revealed 
      that HECn on human neutrophils inhibited a dose-dependent manner the 
      CXCL1-induced neutrophil chemotaxis. Furthermore, HECn also inhibited the 
      chemoattraction of human neutrophils induced by 
      formyl-methionyl-leucyl-phenylalanine (fMLP), leukotriene B4 (LTB4) and platelet 
      activating factor (PAF) in a Boyden chamber. However, this same treatment not was 
      able to induce apoptosis. The results obtained in this study showed that the 
      extract from leaves of C. nemorosa possess a potent inhibitory activity in acute 
      model of inflammation, being the effects mediated, in part, by inhibition of 
      neutrophil responsiveness. These results indicate that C. nemorosa could be a 
      good source for anti-inflammatory compounds.
FAU - Farias, Jose Alex C
AU  - Farias JA
AD  - Laboratorio de Biologia Celular, Instituto de Ciencias Biologicas e da Saude, 
      Universidade Federal de Alagoas, Maceio, Alagoas, Brazil.
FAU - Ferro, Jamylle Nunes S
AU  - Ferro JN
FAU - Silva, Juliane P
AU  - Silva JP
FAU - Agra, Isabela Karine R
AU  - Agra IK
FAU - Oliveira, Fernando M
AU  - Oliveira FM
FAU - Candea, Andre Luiz P
AU  - Candea AL
FAU - Conte, Fernando P
AU  - Conte FP
FAU - Ferraris, Fausto K
AU  - Ferraris FK
FAU - Henriques, Maria das Gracas M O
AU  - Henriques Md
FAU - Conserva, Lucia M
AU  - Conserva LM
FAU - Barreto, Emiliano
AU  - Barreto E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9000-07-1 (Carrageenan)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Carrageenan
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte/drug effects
MH  - *Clusia
MH  - Cotton Fiber
MH  - Granuloma/chemically induced/*drug therapy/immunology
MH  - Humans
MH  - Mice
MH  - Neutrophils/immunology/physiology
MH  - Phytotherapy
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves
MH  - Pleurisy/chemically induced/*drug therapy/immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2011/08/16 06:00
MHDA- 2012/08/29 06:00
CRDT- 2011/08/16 06:00
PHST- 2011/08/16 06:00 [entrez]
PHST- 2011/08/16 06:00 [pubmed]
PHST- 2012/08/29 06:00 [medline]
AID - 10.1007/s10753-011-9372-y [doi]
PST - ppublish
SO  - Inflammation. 2012 Apr;35(2):764-71. doi: 10.1007/s10753-011-9372-y.