PMID- 21843294
OWN - NLM
STAT- MEDLINE
DCOM- 20111212
LR  - 20110816
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Linking)
VI  - 35
IP  - 8
DP  - 2011 Aug
TI  - Alterations in red blood cell volume and hemoglobin concentration, viscoelastic 
      properties, and mechanical fragility caused by continuous flow pumping in calves.
PG  - 791-9
LID - 10.1111/j.1525-1594.2011.01317.x [doi]
AB  - In this study, we have analyzed the changes in mean corpuscular volume (MCV), 
      mean corpuscular hemoglobin concentration (MCHC), and the dynamic deformability 
      and mechanical fragility of red blood cells (RBCs) in five male Holstein calves 
      (body weight: 95.6 +/- 10.8 kg) whose circulation was partially supported with a 
      novel magnetically levitated extracorporeal centrifugal blood pump MedTech Dispo. 
      One hour after the pumping has started, the MCV increased and the MCHC decreased 
      by 1.064 +/- 0.006 and 0.906 +/- 0.050 times, respectively, as compared with those of 
      the prepumped blood (P < 0.05). The deformability index L/W, where L and W are 
      the long and short axes of the two-dimensional RBC images, respectively, sheared 
      by a cyclically reversing shear flow increased indicating that the RBCs pumped 
      for 1 h exhibited more elastic characteristics (P < 0.05). In addition, when the 
      pumped blood cells were sheared for 30 min with a uniform shear stress of 25.38 
      Pa, the hemolysis level decreased dramatically as compared with the control 
      blood, as more fragile RBCs were destroyed by pumping, leaving behind less 
      fragile RBCs. All these characteristics of the RBCs exposed to continuous flow 
      resemble those of young RBCs having larger MCV, lower MCHC, higher elasticity, 
      and lower fragility. In conclusion, during continuous flow pumping, the RBCs 
      having relatively lower threshold for hemolysis to mechanical shear stress 
      generated by continuous flow blood pump (CFBP) are destroyed first and removed 
      from circulation in the early stage of application of CFBP, thus leaving behind 
      less fragile and stronger RBCs.
CI  - (c) 2011, Copyright the Authors. Artificial Organs (c) 2011, International Center for 
      Artificial Organs and Transplantation and Wiley Periodicals, Inc.
FAU - Yokoyama, Naoyuki
AU  - Yokoyama N
AD  - Department of Artificial Organs, Tokyo Medical and Dental University Graduate 
      School of Medical and Dental Sciences, Tokyo, Japan.
FAU - Sakota, Daisuke
AU  - Sakota D
FAU - Nagaoka, Eiki
AU  - Nagaoka E
FAU - Takatani, Setsuo
AU  - Takatani S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Elasticity
MH  - *Erythrocyte Deformability
MH  - *Erythrocyte Indices
MH  - *Erythrocyte Volume
MH  - Erythrocytes/*cytology
MH  - *Extracorporeal Circulation/instrumentation
MH  - Hematocrit
MH  - Male
MH  - Stress, Mechanical
EDAT- 2011/08/17 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/08/17 06:00
PHST- 2011/08/17 06:00 [entrez]
PHST- 2011/08/17 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - 10.1111/j.1525-1594.2011.01317.x [doi]
PST - ppublish
SO  - Artif Organs. 2011 Aug;35(8):791-9. doi: 10.1111/j.1525-1594.2011.01317.x.