PMID- 21844579
OWN - NLM
STAT- MEDLINE
DCOM- 20120125
LR  - 20110816
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 41
IP  - 2
DP  - 2011 Spring
TI  - The utility of fluorescence in situ hybridization (FISH) analysis in diagnosing 
      graft versus host disease following orthotopic liver transplant.
PG  - 188-92
AB  - Graft versus host disease (GVHD) following liver transplant occurs in 0.1-2% of 
      patients and portends a poor prognosis. Affected organs include skin, the 
      gastrointestinal tract and bone marrow. We present the case of a 61 year old 
      female who developed skin rash and pancytopenia following sex-mismatched second 
      liver transplant for autoimmune hepatitis. Initial skin biopsies revealed 
      vacuolar interface change, keratinocyte necrosis and a mild mononuclear 
      superficial perivascular infiltrate. The bone marrow was markedly hypocellular 
      with scattered CD8 positive T lymphocytes. FISH analysis revealed chimerism with 
      the presence of male donor cells in the skin and bone marrow biopsies. This case 
      illustrates the diagnostic utility of FISH in detecting the presence of 
      donor-derived cells in tissues affected by GVHD.
FAU - Post, Ginell R
AU  - Post GR
AD  - Department of Pathology, University of Arkansas for Medical Sciences, 4301 W. 
      Markham Street, Little Rock, AR 72205-7199, USA. gpost@uams.edu
FAU - Black, Joshua S
AU  - Black JS
FAU - Cortes, Gil Y
AU  - Cortes GY
FAU - Pollack, Ross Brian
AU  - Pollack RB
FAU - Wolff, Daynna J
AU  - Wolff DJ
FAU - Lazarchick, John
AU  - Lazarchick J
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
SB  - IM
MH  - Bone Marrow Cells/pathology
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - Chimerism
MH  - Female
MH  - Graft vs Host Disease/*diagnosis/etiology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Keratinocytes/pathology
MH  - Liver Transplantation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Pancytopenia/etiology/pathology
MH  - Skin/pathology
EDAT- 2011/08/17 06:00
MHDA- 2012/01/26 06:00
CRDT- 2011/08/17 06:00
PHST- 2011/08/17 06:00 [entrez]
PHST- 2011/08/17 06:00 [pubmed]
PHST- 2012/01/26 06:00 [medline]
AID - 41/2/188 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2011 Spring;41(2):188-92.