PMID- 21845039
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR  - 20211020
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 6
DP  - 2011
TI  - Nickel nanowires induced and reactive oxygen species mediated apoptosis in human 
      pancreatic adenocarcinoma cells.
PG  - 1475-85
LID - 10.2147/IJN.S21697 [doi]
AB  - BACKGROUND: The ability to evade apoptosis is one of the key properties of 
      cancer. The apoptogenic effect of nickel nanowires (Ni NWs) on cancer cell lines 
      has never been adequately addressed. Due to the unique physicochemical 
      characteristics of Ni NWs, we envision the development of a novel anticancer 
      therapeutics specifically for pancreatic cancer. Thus, we investigated whether Ni 
      NWs induce ROS-mediated apoptosis in human pancreatic adenocarcinoma (Panc-1) 
      cells. METHODS: In this study Ni NWs were fabricated using the electrodeposition 
      method. Synthesized Ni NWs were physically characterized by energy dispersive 
      X-ray analysis, UV-Vis spectroscopy of NanoDrop 2000 (UV-Vis), magnetization 
      study, scanning electron microscopy, and transmission electron microscopy. 
      Assessment of morphological apoptotic characteristics by phase contrast 
      microscopy (PCM), Ni-NWs-induced apoptosis staining with ethidium bromide (EB) 
      and acridine orange (AO) followed by fluorescence microscopy (FM) was performed. 
      For molecular biological and biochemical characterization, Panc-1 cell culture 
      and cytotoxic effect of Ni NWs were determined by using 3-(4, 
      5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. 
      Quantitative apoptosis was analyzed by flow cytometry staining with propidium 
      iodide through cell cycle arrest and generation of ROS using 2', 
      7'-dichlorofluorescein diacetate fluorescence intensity. In all experiments, 
      Panc-1 cancer cells without any treatment were used as the negative controls. 
      RESULTS: The intracellular uptake of Ni NWs through endocytosis by Panc-1 cells 
      was observed by PCM. EB and AO staining of FM and MTT assay qualitatively and 
      quantitatively confirmed the extent of apoptosis. Flow cytometric cell cycle 
      arrest and ROS generation indicated Ni NWs as inducers of apoptotic cell death. 
      CONCLUSION: We investigated the role of Ni NWs as inducers of ROS-mediated 
      apoptosis in Panc-1 cells. These results suggested that Ni NWs could be an 
      effective apoptotic agent for Panc-1 cells and have good potential for further 
      research into a clinical treatment selective for pancreatic cancer.
FAU - Hossain, Md Zakir
AU  - Hossain MZ
AD  - Applied Biosciences-Bionanotechnology Research, Department of Applied Science, 
      University of Arkansas at Little Rock, Little Rock, Arkansas 72204, USA. 
      mxhossain1@ualr.edu
FAU - Kleve, Maurice G
AU  - Kleve MG
LA  - eng
PT  - Journal Article
DEP - 20110713
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 7OV03QG267 (Nickel)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Adenocarcinoma/*drug therapy/metabolism/pathology
MH  - Analysis of Variance
MH  - Apoptosis/*drug effects
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Flow Cytometry
MH  - Fluorescent Dyes
MH  - Humans
MH  - Microscopy, Phase-Contrast
MH  - Nanotechnology
MH  - Nanowires/*chemistry
MH  - Nickel/chemistry/pharmacokinetics/*pharmacology
MH  - Pancreatic Neoplasms/*drug therapy/metabolism/pathology
MH  - Particle Size
MH  - Reactive Oxygen Species/*metabolism
MH  - Tetrazolium Salts
MH  - Thiazoles
PMC - PMC3152467
OTO - NOTNLM
OT  - MTT assay
OT  - Ni NWs
OT  - Panc-1
OT  - ROS
OT  - apoptosis
OT  - cell cycle arrest
OT  - flow cytometry
EDAT- 2011/08/17 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/13
CRDT- 2011/08/17 06:00
PHST- 2011/08/17 06:00 [entrez]
PHST- 2011/08/17 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/13 00:00 [pmc-release]
AID - ijn-6-1475 [pii]
AID - 10.2147/IJN.S21697 [doi]
PST - ppublish
SO  - Int J Nanomedicine. 2011;6:1475-85. doi: 10.2147/IJN.S21697. Epub 2011 Jul 13.