PMID- 21845146
OWN - NLM
STAT- MEDLINE
DCOM- 20111219
LR  - 20240109
IS  - 1660-4601 (Electronic)
IS  - 1661-7827 (Print)
IS  - 1660-4601 (Linking)
VI  - 8
IP  - 7
DP  - 2011 Jul
TI  - Pulsed ultraviolet light reduces immunoglobulin E binding to Atlantic white 
      shrimp (Litopenaeus setiferus) extract.
PG  - 2569-83
LID - 10.3390/ijerph8072569 [doi]
AB  - Pulsed ultraviolet light (PUV), a novel food processing and preservation 
      technology, has been shown to reduce allergen levels in peanut and soybean 
      samples. In this study, the efficacy of using PUV to reduce the reactivity of the 
      major shrimp allergen, tropomyosin (36-kDa), and to attenuate immunoglobulin E 
      (IgE) binding to shrimp extract was examined. Atlantic white shrimp (Litopenaeus 
      setiferus) extract was treated with PUV (3 pulses/s, 10 cm from light source) for 
      4 min. Tropomyosin was compared in the untreated, boiled, PUV-treated and 
      [boiled+PUV]-treated samples, and changes in the tropomyosin levels were 
      determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis 
      (SDS-PAGE). IgE binding of the treated extract was analyzed via immunoblot and 
      enzyme-linked immunosorbent assay (ELISA) using pooled human plasma containing 
      IgE antibodies against shrimp allergens. Results showed that levels of 
      tropomyosin and IgE binding were reduced following PUV treatment. However, 
      boiling increased IgE binding, while PUV treatment could offset the increased 
      allergen reactivity caused by boiling. In conclusion, PUV treatment reduced the 
      reactivity of the major shrimp allergen, tropomyosin, and decreased the IgE 
      binding capacity of the shrimp extract.
FAU - Shriver, Sandra
AU  - Shriver S
AD  - Department of Food Science & Human Nutrition, University of Florida, P.O. Box 
      110370, 359 FSHN Bldg. Newell Drive, Gainesville, FL 32611, USA. 
      sandrakshriver@ufl.edu
FAU - Yang, Wade
AU  - Yang W
FAU - Chung, Si-Yin
AU  - Chung SY
FAU - Percival, Susan
AU  - Percival S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20110624
PL  - Switzerland
TA  - Int J Environ Res Public Health
JT  - International journal of environmental research and public health
JID - 101238455
RN  - 0 (Allergens)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology/radiation effects
MH  - Animals
MH  - Arthropod Proteins/immunology/*radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Food Handling/*methods
MH  - Food Hypersensitivity/immunology/*prevention & control
MH  - Food Preservation/instrumentation/methods
MH  - Humans
MH  - Immunoglobulin E/metabolism
MH  - Penaeidae/*chemistry/immunology
MH  - Shellfish/*radiation effects
MH  - Solubility
MH  - Tropomyosin/immunology/*radiation effects
MH  - *Ultraviolet Rays
PMC - PMC3155317
OTO - NOTNLM
OT  - IgE antibodies
OT  - PUV
OT  - allergen
OT  - allergy
OT  - pulsed ultraviolet light
OT  - shrimp
OT  - tropomyosin
EDAT- 2011/08/17 06:00
MHDA- 2011/12/20 06:00
PMCR- 2011/07/01
CRDT- 2011/08/17 06:00
PHST- 2011/05/18 00:00 [received]
PHST- 2011/06/19 00:00 [accepted]
PHST- 2011/08/17 06:00 [entrez]
PHST- 2011/08/17 06:00 [pubmed]
PHST- 2011/12/20 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - ijerph8072569 [pii]
AID - ijerph-08-02569 [pii]
AID - 10.3390/ijerph8072569 [doi]
PST - ppublish
SO  - Int J Environ Res Public Health. 2011 Jul;8(7):2569-83. doi: 
      10.3390/ijerph8072569. Epub 2011 Jun 24.