PMID- 21846147
OWN - NLM
STAT- MEDLINE
DCOM- 20120113
LR  - 20110921
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 59
IP  - 18
DP  - 2011 Sep 28
TI  - Human immunoglobulin E (IgE) binding to heated and glycated ovalbumin and 
      ovomucoid before and after in vitro digestion.
PG  - 10044-51
LID - 10.1021/jf2014638 [doi]
AB  - This study focuses on the effect of heating and Maillard reaction (MR) on the in 
      vitro digestibility and rabbit IgG- and human IgE-binding properties of ovalbumin 
      (OVA) and ovomucoid (OM) to estimate the impact of processing on their 
      allergenicity. With the human sera studied, heat treatment significantly reduced 
      IgE binding to both OVA and OM, whereas MR reduced the IgE binding to OVA but 
      increased IgE binding to OM. In contrast, heat treatment significantly favored 
      OVA digestibility but glycation impaired it, and these treatments did not affect 
      the digestibility of OM. The changes observed in the digestibility affected the 
      immunogenicity of the digests accordingly, so that the higher the digestibility, 
      the lower the antibody binding. Heat treatment and glycation by MR showed an 
      influence on the potential allergenicity of the main egg white proteins that 
      could be related to their resistance to denaturation and digestive enzymes.
FAU - Jimenez-Saiz, Rodrigo
AU  - Jimenez-Saiz R
AD  - Institute of Food Science Research (CIAL) CSIC-UAM, Universidad Autonoma de 
      Madrid, C/Nicolas Cabrera 9, 28049 Madrid, Spain.
FAU - Belloque, Josefina
AU  - Belloque J
FAU - Molina, Elena
AU  - Molina E
FAU - Lopez-Fandino, Rosina
AU  - Lopez-Fandino R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110826
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 37281-36-0 (Ovomucin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - *Digestion
MH  - Food Hypersensitivity/immunology
MH  - Glycosylation
MH  - *Hot Temperature
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Maillard Reaction
MH  - Ovalbumin/chemistry/*immunology/metabolism
MH  - Ovomucin/chemistry/*immunology/metabolism
MH  - Rabbits
EDAT- 2011/08/19 06:00
MHDA- 2012/01/14 06:00
CRDT- 2011/08/18 06:00
PHST- 2011/08/18 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2012/01/14 06:00 [medline]
AID - 10.1021/jf2014638 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2011 Sep 28;59(18):10044-51. doi: 10.1021/jf2014638. Epub 2011 
      Aug 26.