PMID- 21846913
OWN - NLM
STAT- MEDLINE
DCOM- 20111031
LR  - 20191210
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 136
IP  - 3
DP  - 2011 Sep
TI  - Evaluation of a selection strategy before use of 16S rRNA gene sequencing for the 
      identification of clinically significant gram-negative rods and coccobacilli.
PG  - 381-8
LID - 10.1309/AJCP61CGNXCXVSPR [doi]
AB  - Although 16S ribosomal RNA (rRNA) gene sequencing is well established for 
      correctly identifying bacteria, its most efficient use in a routine clinical 
      laboratory is not clear. We devised and evaluated a strategy to select 
      gram-negative rods and coccobacilli (GNRCB) for which sequencing might be 
      necessary before routine identification methods had been exhausted. The 
      prospectively applied selection criteria were primarily based on the isolate's 
      display of unusual or discordant phenotypic results and/or disease correlation. 
      By using this strategy, we selected a total of 120 GNRCB (representing only  approximately 2% 
      of all identified). The strategy was demonstrated to be efficient because the 
      preliminary phenotypic identification for 79.2% of those isolates needed revision 
      (18.2% were novel and about a third would have required further extensive 
      testing). The knowledge that 1.6% (ie, 79% of 2%) of isolated GNRCB might benefit 
      from sequence identification could provide guidelines for routine clinical 
      laboratories toward efficient use of sequence analysis.
FAU - Mahlen, Steven D
AU  - Mahlen SD
AD  - Department of Laboratory Medicine, University of Washington, Seattle, USA.
FAU - Clarridge, Jill E 3rd
AU  - Clarridge JE 3rd
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacterial Typing Techniques/*methods
MH  - DNA, Bacterial/chemistry
MH  - Gram-Negative Bacteria/*classification/genetics/isolation & purification
MH  - Humans
MH  - Prospective Studies
MH  - RNA, Bacterial/chemistry
MH  - RNA, Ribosomal, 16S/*genetics
EDAT- 2011/08/19 06:00
MHDA- 2011/11/01 06:00
CRDT- 2011/08/18 06:00
PHST- 2011/08/18 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2011/11/01 06:00 [medline]
AID - 136/3/381 [pii]
AID - 10.1309/AJCP61CGNXCXVSPR [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2011 Sep;136(3):381-8. doi: 10.1309/AJCP61CGNXCXVSPR.