PMID- 21846921
OWN - NLM
STAT- MEDLINE
DCOM- 20111031
LR  - 20110817
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 136
IP  - 3
DP  - 2011 Sep
TI  - Identification of malignant cytologic criteria in pancreatobiliary brushings with 
      corresponding positive fluorescence in situ hybridization results.
PG  - 442-9
LID - 10.1309/AJCPDULIOEOTUZ5H [doi]
AB  - Cytologic evaluation of pancreatobiliary brushings is specific but poorly 
      sensitive for malignancy. Detection of polysomic cells by fluorescence in situ 
      hybridization (FISH) is significantly more sensitive than routine cytology with 
      similar specificity. The purpose of this study was to identify cytologic criteria 
      most associated with malignancy in specimens unaffected by sample failure. 
      Endoscopic brushings were split equally for routine cytologic and FISH analyses 
      per clinical practice. We retrospectively evaluated 16 cytologic criteria on 
      Papanicolaou-stained slides. We assumed that the presence of polysomic cells by 
      FISH indicated successful tumor sampling in specimens from patients with 
      pathologic evidence of malignancy on follow-up. We compared cytologic criteria of 
      malignant brushings with corresponding positive FISH results (positive control, n 
      = 39) with those without evidence of malignancy and corresponding negative FISH 
      results (negative control, n = 30). The presence of single abnormal cells, 
      irregular nuclear membranes, and enlarged nuclei were independent predictors of 
      malignancy by logistic regression (P < .05).
FAU - Barr Fritcher, Emily G
AU  - Barr Fritcher EG
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 
      Rochester, MN 55905, USA.
FAU - Caudill, Jill L
AU  - Caudill JL
FAU - Blue, Joshua E
AU  - Blue JE
FAU - Djuric, Kris
AU  - Djuric K
FAU - Feipel, Lesley
AU  - Feipel L
FAU - Maritim, Benard K
AU  - Maritim BK
FAU - Ragheb, Ameera A
AU  - Ragheb AA
FAU - Halling, Kevin C
AU  - Halling KC
FAU - Henry, Michael R
AU  - Henry MR
FAU - Clayton, Amy C
AU  - Clayton AC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Adenocarcinoma/*pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bile Duct Neoplasms/*pathology
MH  - Cholangiocarcinoma/*pathology
MH  - Cholangitis, Sclerosing/*pathology
MH  - Cytodiagnosis/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Neoplasms/*pathology
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
EDAT- 2011/08/19 06:00
MHDA- 2011/11/01 06:00
CRDT- 2011/08/18 06:00
PHST- 2011/08/18 06:00 [entrez]
PHST- 2011/08/19 06:00 [pubmed]
PHST- 2011/11/01 06:00 [medline]
AID - 136/3/442 [pii]
AID - 10.1309/AJCPDULIOEOTUZ5H [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2011 Sep;136(3):442-9. doi: 10.1309/AJCPDULIOEOTUZ5H.